Ombuoside is a glycoside complex extracted from Gynostemma pentaphyllum. Ombuoside has antimicrobial property against several Gram-positive (Gram+) and Gram-negative (Gram+) bacteria as well as Candida albicans. Ombuoside has free radical scavenging and ROS antioxidant effects.

Physicochemical Properties

Molecular Formula	C29H34O16
Molecular Weight	638.57
Exact Mass	638.184
CAS #	20188-85-6
PubChem CID	10100634
Appearance	White to light yellow solid powder
Density	1.7±0.1 g/cm3
Boiling Point	947.6±65.0 °C at 760 mmHg
Flash Point	308.6±27.8 °C
Vapour Pressure	0.0±0.3 mmHg at 25°C
Index of Refraction	1.697
LogP	2.17
Hydrogen Bond Donor Count	8
Hydrogen Bond Acceptor Count	16
Rotatable Bond Count	8
Heavy Atom Count	45
Complexity	1060
Defined Atom Stereocenter Count	10
SMILES	C[C@H]1[C@@H]([C@H]([C@H]([C@@H](O1)OC[C@@H]2[C@H]([C@@H]([C@H]([C@@H](O2)OC3=C(OC4=CC(=CC(=C4C3=O)O)OC)C5=CC(=C(C=C5)OC)O)O)O)O)O)O)O
InChi Key	VVSFMIXQNYRGMG-BDAFLREQSA-N
InChi Code	InChI=1S/C29H34O16/c1-10-19(32)22(35)24(37)28(42-10)41-9-17-20(33)23(36)25(38)29(44-17)45-27-21(34)18-14(31)7-12(39-2)8-16(18)43-26(27)11-4-5-15(40-3)13(30)6-11/h4-8,10,17,19-20,22-25,28-33,35-38H,9H2,1-3H3/t10-,17+,19-,20+,22+,23-,24+,25+,28+,29-/m0/s1
Chemical Name	5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-7-methoxy-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxymethyl]oxan-2-yl]oxychromen-4-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Isolation, Identification and Antimicrobial Activity of Ombuoside From Stevia Triflora. Ann Pharm Fr. 1997;55(6):262-8. [2]. Ombuoside From Gynostemma Pentaphyllum Protects PC12 Cells From L-DOPA-Induced Neurotoxicity.Planta Med.
Additional Infomation	Ombuoside has been reported in Kaempferia parviflora, Gynostemma pentaphyllum, and Bryobium eriaeoides with data available.

Solubility Data

Solubility (In Vitro)

DMSO : 50 mg/mL (78.30 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (1.96 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.5660 mL	7.8300 mL	15.6600 mL
	5 mM	0.3132 mL	1.5660 mL	3.1320 mL
	10 mM	0.1566 mL	0.7830 mL	1.5660 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.