Octaethylene glycol monododecyl ether (C12E8) is a nonionic detergent that can be used for membrane protein extraction. Octaethylene glycol monododecyl ether can dissolve the viral membrane of intact influenza viruses.

Physicochemical Properties

Molecular Formula	C28H58O9
Molecular Weight	538.75
Exact Mass	538.408
CAS #	3055-98-9
PubChem CID	123921
Appearance	Colorless to light yellow liquid
Density	1.0±0.1 g/cm3
Boiling Point	585.5±45.0 °C at 760 mmHg
Melting Point	30ºC
Flash Point	307.9±28.7 °C
Vapour Pressure	0.0±3.7 mmHg at 25°C
Index of Refraction	1.459
LogP	2.54
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	9
Rotatable Bond Count	34
Heavy Atom Count	37
Complexity	389
Defined Atom Stereocenter Count	0
SMILES	CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCO
InChi Key	YYELLDKEOUKVIQ-UHFFFAOYSA-N
InChi Code	InChI=1S/C28H58O9/c1-2-3-4-5-6-7-8-9-10-11-13-30-15-17-32-19-21-34-23-25-36-27-28-37-26-24-35-22-20-33-18-16-31-14-12-29/h29H,2-28H2,1H3
Chemical Name	2-[2-[2-[2-[2-[2-[2-(2-dodecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	It has been demonstrated that intact influenza virus membranes can be solubilized by octaethylene glycol monododecyl ether (C12E8). The accumulation of C12E8 molecules outside the membrane until micelle formation, which allows for the extraction of membrane contents, provides the basis for the mechanism of viral membrane solubilization[1].
References	[1]. Aqueous solutions of didecyldimethylammonium chloride and octaethylene glycol monododecyl ether: Toward synergistic formulations against enveloped viruses. Int J Pharm. 2016 Sep 10;511(1):550-559. [2]. Characterization of mixed non-ionic surfactants n-octyl-β-D-thioglucoside and octaethylene-glycol monododecyl ether: micellization and microstructure. J Colloid Interface Sci. 2011 Sep 1;361(1):178-85.
Additional Infomation	Octaethyleneglycol monododecyl ether is the hydroxypolyether that is octaethylene glycol in which one of the hydroxy groups is substituted by dodecyloxy. It is functionally related to an octaethylene glycol.

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (185.61 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (4.64 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.64 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (4.64 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.8561 mL	9.2807 mL	18.5615 mL
	5 mM	0.3712 mL	1.8561 mL	3.7123 mL
	10 mM	0.1856 mL	0.9281 mL	1.8561 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.