OVA Peptide(257-264) TFA is the class I (Kb)-restricted peptide epitope of OVA, which is an octamer peptide that can be developed from ovalbumin presented by the class I MHC molecule H-2Kb.

Physicochemical Properties

Molecular Formula	C49H76F6N10O17
Molecular Weight	1191.17497444153
Exact Mass	1076.536
CAS #	1262751-08-5
Related CAS #	OVA Peptide(257-264);138831-86-4;OVA Peptide(257-264) acetate salt
PubChem CID	71311993
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	14
Hydrogen Bond Acceptor Count	20
Rotatable Bond Count	33
Heavy Atom Count	75
Complexity	1780
Defined Atom Stereocenter Count	10
SMILES	FC(C(=O)O)(F)F.FC(C(=O)O)(F)F.O=C([C@H]([C@@H](C)CC)NC([C@H](CO)N)=O)N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(=O)O)CC(C)C)=O)CCCCN)=O)CCC(=O)O)=O)CC1C=CC=CC=1)=O)CC(N)=O)=O)[C@@H](C)CC
InChi Key	BRUCWCULSHVYBQ-VPZPQSBKSA-N
InChi Code	InChI=1S/C45H74N10O13.C2HF3O2/c1-7-25(5)36(55-44(66)37(26(6)8-2)54-38(60)28(47)23-56)43(65)52-32(22-34(48)57)42(64)51-31(21-27-14-10-9-11-15-27)41(63)50-30(17-18-35(58)59)40(62)49-29(16-12-13-19-46)39(61)53-33(45(67)68)20-24(3)4;3-2(4,5)1(6)7/h9-11,14-15,24-26,28-33,36-37,56H,7-8,12-13,16-23,46-47H2,1-6H3,(H2,48,57)(H,49,62)(H,50,63)(H,51,64)(H,52,65)(H,53,61)(H,54,60)(H,55,66)(H,58,59)(H,67,68);(H,6,7)/t25-,26-,28-,29-,30-,31-,32-,33-,36-,37-;/m0./s1
Chemical Name	(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-4-amino-2-[[(2S,3S)-2-[[(2S,3S)-2-[[(2S)-2-amino-3-hydroxypropanoyl]amino]-3-methylpentanoyl]amino]-3-methylpentanoyl]amino]-4-oxobutanoyl]amino]-3-phenylpropanoyl]amino]-4-carboxybutanoyl]amino]hexanoyl]amino]-4-methylpentanoic acid;2,2,2-trifluoroacetic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

TAP1-I- and C57BL/6 macrophages can process Crl-OVA and full-length OVA in distinct cellular compartments. Additionally, TAP-independent processing of MHC class I presentation is influenced by the protein background of the OVA epitope, OVA peptide (257-264). The TAP transporter presents the epitope in several ways: While the OVA peptide (257–264) in full-length ovalbumin is highly dependent on the TAP transporter, regardless of whether the native protein is coupled to polystyrene or the recombinant OVA is expressed in bacteria, the OVA peptide (257–264) in the MBPCrl-OVA or Crl-OVA bacterial fusion protein depends on the TAP transporter [1].

References

[1]. Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation. Eur J Immunol. 1996 Nov;26(11):2790-9.

Solubility Data

Solubility (In Vitro)

DMSO : ~125 mg/mL (~116.05 mM) H2O : ~50 mg/mL (~46.42 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (1.93 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.8395 mL	4.1975 mL	8.3950 mL
	5 mM	0.1679 mL	0.8395 mL	1.6790 mL
	10 mM	0.0840 mL	0.4198 mL	0.8395 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.