Neurodazine is a neurogenic inducer that works as a promoter of neurogenesis in pluripotent cells. Neurodazine promotes neurogenesis by activating Wnt and Shh signaling pathways.

Physicochemical Properties

Molecular Formula	C27H25CLN2O3
Molecular Weight	460.95200
Exact Mass	460.155
CAS #	937807-66-4
PubChem CID	16112820
Appearance	Light yellow to yellow solid powder
Density	1.2±0.1 g/cm3
Boiling Point	669.0±55.0 °C at 760 mmHg
Flash Point	358.4±31.5 °C
Vapour Pressure	0.0±2.0 mmHg at 25°C
Index of Refraction	1.613
LogP	6.42
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	6
Heavy Atom Count	33
Complexity	608
Defined Atom Stereocenter Count	0
InChi Key	FEEOFPAEDSMOTO-UHFFFAOYSA-N
InChi Code	InChI=1S/C27H21ClN2O3/c1-31-21-10-6-17(7-11-21)25-26(18-8-12-22(32-2)13-9-18)30-27(29-25)24-15-14-23(33-24)19-4-3-5-20(28)16-19/h3-16H,1-2H3,(H,29,30)
Chemical Name	2-[5-(3-chlorophenyl)furan-2-yl]-4,5-bis(4-methoxyphenyl)-1H-imidazole
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	P19 cells treated with neurodazine (5 μM; 1–10 days) exhibit markers unique to neurons [1]. Neurodazine specifically prevents P19 cells from differentiating into astrocytes [1].
Cell Assay	Western Blot Analysis[1] Cell Types: P19 Cell Tested Concentrations: 5 μM Incubation Duration: 1-10 days Experimental Results: Induced expression of neuron-specific markers.
References	[1]. Imidazole-based small molecules that promote neurogenesis in pluripotent cells. Angew Chem Int Ed Engl. 2014 Aug 25;53(35):9271-4. [2]. Synthetic small molecules that induce neuronal differentiation in neuroblastoma and fibroblast cells. Mol Biosyst. 2015 Oct;11(10):2727-37.
Additional Infomation	2-[5-(3-chlorophenyl)-2-furanyl]-4,5-bis(4-methoxyphenyl)-1H-imidazole is a member of imidazoles.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~218.86 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (5.47 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (5.47 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1694 mL	10.8472 mL	21.6943 mL
	5 mM	0.4339 mL	2.1694 mL	4.3389 mL
	10 mM	0.2169 mL	1.0847 mL	2.1694 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.