Neuraminidase-IN-1， also known as Y-1, is a neuraminidase inhibitor, with an IC50 of 0.21 μM. Neuraminidase-IN-1 has excellent activity against H1N1 influenza virus and good inhibitory effect on the A/WSN/33 H1N1 virus strains.Y-1 exerts the best inhibition activity (IC50 = 0.21 μM) against NA, which is better than oseltamivir carboxylate (OSC) (IC50 = 3.04 μM) and lead AN-329/10738021 (IC50 = 1.92 μM).

Physicochemical Properties

Molecular Formula	C14H11N3O6
Molecular Weight	317.253643274307
Exact Mass	317.06
Elemental Analysis	C, 53.00; H, 3.49; N, 13.25; O, 30.26
CAS #	2379438-80-7
PubChem CID	154585120
Appearance	Light yellow to yellow solid powder
LogP	2.2
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	3
Heavy Atom Count	23
Complexity	456
Defined Atom Stereocenter Count	0
SMILES	C1=CC(=C(C=C1/C=N/NC(=O)C2=CC(=CC(=C2)O)O)[N+](=O)[O-])O
InChi Key	MCGROFKAAXXTBN-VIZOYTHASA-N
InChi Code	InChI=1S/C14H11N3O6/c18-10-4-9(5-11(19)6-10)14(21)16-15-7-8-1-2-13(20)12(3-8)17(22)23/h1-7,18-20H,(H,16,21)/b15-7+
Chemical Name	Benzoic acid, 3,5-dihydroxy-, (2E)-2-[(4-hydroxy-3-nitrophenyl)methylene]hydrazide
Synonyms	MVN38807; MVN-38807; MVN 38807;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Neuraminidase-IN-1 exhibits low toxicity, good drugability, and a good inhibitory activity against the A/WSN/33H1N1 virus strain [1].
References	[1]. Preparation of dihydroxybenzoylhydrazone neuraminidase inhibitor used in preparation of drugs for resisting H1N1 influenza virus. CN109776354A.

Solubility Data

Solubility (In Vitro)

DMSO : ~125 mg/mL (~394.01 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.08 mg/mL (6.56 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.08 mg/mL (6.56 mM)  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.1521 mL	15.7604 mL	31.5209 mL
	5 mM	0.6304 mL	3.1521 mL	6.3042 mL
	10 mM	0.3152 mL	1.5760 mL	3.1521 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.