Neplanocin A ((-)-Neplanocin A) is an antitumor antibiotic with significant antitumor activity against mouse L1210 leukemia. Neplanocin A is also an irreversible AdoHcy hydrolase inhibitor (Ki=8.39 nM). Neplanocin A also has antiviral activity and can effectively inhibit vaccinia virus. Neplanocin A can be obtained from Ampullariella regularis.

Physicochemical Properties

Molecular Formula	C11H13N5O3
Molecular Weight	263.25
CAS #	72877-50-0
Appearance	White to off-white solid powder
SMILES	C1=C(C(C(C1N2C=NC3=C(N=CN=C32)N)O)O)CO
Synonyms	(-)-Neplanocin A
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	AdoHcy hydrolase; vaccinia[1].
ln Vitro	Neplanocin A (1 μM; 0-70 h) inhibits the growth of mouse L929 cells [1]. Neplanocin A (1 μM; 72 h) inhibits the formation of vaccinia virus plaques in mouse L929 cell monolayer cultures [1].
Cell Assay	Cell Viability Assay[1] Cell Types: L929 mouse cells Tested Tested Concentrations: 1 μM Incubation Duration: 0-70 h Experimental Results: Slowed the rate of increase in cell number.
References	[1]. Borchardt RT, Keller BT, Patel-Thombre U. Neplanocin A. A potent inhibitor of S-adenosylhomocysteine hydrolase and of vaccinia virus multiplication in mouse L929 cells. J Biol Chem. 1984 Apr 10;259(7):4353-8.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.7987 mL	18.9934 mL	37.9867 mL
	5 mM	0.7597 mL	3.7987 mL	7.5973 mL
	10 mM	0.3799 mL	1.8993 mL	3.7987 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.