Physicochemical Properties
| Exact Mass | 516.087 |
| CAS # | 133876-92-3 |
| Appearance | White to off-white solid powder |
| Density | 1.5±0.1 g/cm3 |
| Index of Refraction | 1.580 |
| SMILES | ClC1C([H])=C([H])C(=C([H])C=1C(N([H])C([H])([H])C(N([H])[C@]([H])(B1OC(C([H])([H])C(C(=O)O[H])(C([H])([H])C(=O)O[H])O1)=O)C([H])([H])C([H])(C([H])([H])[H])C([H])([H])[H])=O)=O)Cl |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | Ninety-four percent of blood clots disintegrate in ten minutes when nattokinase is added, and fibrin clots breakdown after two hours [2]. |
| ln Vivo | In vivo, nattokinase (150–250 mg/kg; orally administered twice daily for two days) demonstrates fibrinolytic activity and breaks up blood clots[3]. |
| Animal Protocol |
Animal/Disease Models: SD (Sprague-Dawley) rats (180-220 g) are injected κ-Carrageenan[3] Doses: 150, 250 mg/kg Route of Administration: po (oral gavage) twice (two times) daily for 2 days Experimental Results: Increased the plasma concentration of fibrin/ fibrinogen degradation products (FDPs) and D-Dimer in a dose-dependent manner. diminished the fraction of the vessel cross section occupied by thrombosis. |
| References |
[1]. Nattokinase: An Oral Antithrombotic Agent for the Prevention of Cardiovascular Disease. Int J Mol Sci. 2017 Feb 28; 18(3):523. [2]. Exploring the In Vitro Thrombolytic Activity of Nattokinase From a New Strain Pseudomonas aeruginosa CMSS. Jundishapur J Microbiol. 2015 Oct 26; 8(10): e23567. [3]. Thrombolytic effects in vivo of nattokinase in a carrageenan-induced rat model of thrombosis. Acta Haematol. 2014; 132(2): 247-53. |
Solubility Data
| Solubility (In Vitro) | H2O: 100 mg/mL |
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication. (Please use freshly prepared in vivo formulations for optimal results.) |