Naftazone (Etioven) is a novel and potent vasoprotectant drug used for hemostasis. It can accelerate human saphenous vein endothelial cell proliferation in vitro at concentrations which did not alter the hemostatic balance.
Physicochemical Properties
| Molecular Formula | C11H9N3O2 |
| Molecular Weight | 215.212 |
| Exact Mass | 215.069 |
| CAS # | 15687-37-3 |
| PubChem CID | 71688 |
| Appearance | Yellow to orange solid powder |
| Density | 1.4g/cm3 |
| Index of Refraction | 1.678 |
| LogP | 2.011 |
| Hydrogen Bond Donor Count | 2 |
| Hydrogen Bond Acceptor Count | 3 |
| Rotatable Bond Count | 1 |
| Heavy Atom Count | 16 |
| Complexity | 293 |
| Defined Atom Stereocenter Count | 0 |
| SMILES | NC(N/N=C1\C=CC2=CC=CC=C2C\1=O)=O |
| InChi Key | AGSIRJFXAANBMW-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C11H9N3O2/c12-11(16)14-13-9-6-5-7-3-1-2-4-8(7)10(9)15/h1-6,15H,(H2,12,16) |
| Chemical Name | (1-hydroxynaphthalen-2-yl)iminourea |
| Synonyms | C05CX02 Etioven Naftazone |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | In mouse cerebellar synaptosomes, naftazone (0.5-50 μM; 1 h) decreases glutamate release[1]. |
| ln Vivo | The glutamate content of the cerebrospinal fluid is impacted by naftazone (10 and 100 mg/kg; po once daily for 15 days)[1]. Rats' platelet function is impacted by naftazone (50 mg/kg; intraperitoneally once daily for five days)[2]. |
| Animal Protocol |
Animal/Disease Models: Male rats[1] Doses: 10 and 100 mg/kg Route of Administration: po (oral gavage); 10 and 100 mg/kg once per day; for 15 days Experimental Results: Dramatically decreased glutamate content in cerebro spinal fluid of rats. Animal/Disease Models: Wistar rats[2] Doses: 50 mg/kg Route of Administration: intraperitoneal (ip)injection ; 50 mg/kg one time/day for 5 days Experimental Results: decreased the height of platelet aggregation induced by ADP, Dramatically increased the platelet disaggregation induced by collagen and decreased fibrinogen binding to 2.5 or 5 μM ADP-stimulated platelet. |
| References |
[1]. Naftazone reduces glutamate cerebro spinal fluid levels in rats and glutamate release from mouse cerebellum synaptosomes. Neurosci Lett. 1999 Aug 27;271(3):183-6. [2]. Effect of naftazone on in vivo platelet function in the rat. Platelets. 1999;10(1):66-70. |
| Additional Infomation | Naftazone is a member of naphthalenes. |
Solubility Data
| Solubility (In Vitro) | May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6466 mL | 23.2331 mL | 46.4662 mL | |
| 5 mM | 0.9293 mL | 4.6466 mL | 9.2932 mL | |
| 10 mM | 0.4647 mL | 2.3233 mL | 4.6466 mL |