NZ-804 is an orally active inhibitor of the major SARS-CoV-2 protease Mpro with an IC50 of 8.9 nM. NZ-804 inhibits SARS-CoV-2 replication in HeLa-hACE2 cells with an EC50 of 14 nM. NZ-804 exhibits broad-spectrum antiviral activity against multiple CoVs. NZ-804 reduces viral replication in mouse and hamster models.

Physicochemical Properties

Molecular Formula	C27H23N3O3S
Molecular Weight	469.55
CAS #	2858658-44-1
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: 8.9 nM (SARS-CoV-2 Mpro)
References	[1]. An oral non-covalent non-peptidic inhibitor of SARS-CoV-2 Mpro ameliorates viral replication and pathogenesis in vivo. Cell Rep. 2024 Nov 26;43(11):114929.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1297 mL	10.6485 mL	21.2970 mL
	5 mM	0.4259 mL	2.1297 mL	4.2594 mL
	10 mM	0.2130 mL	1.0648 mL	2.1297 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.