SDZ285428 is a CYP51 inhibitor. SDZ285428 inhibits Trypanosoma cruzi (TC) CYP51 with I/E2 <1 (5 min) and I/E2=9 (1 h). SDZ285428 inhibits Trypanosoma brucei (TB) CYP51 with I/E2 <1 (5 min) and I/E2=35 (1 h).

Physicochemical Properties

Molecular Formula	C24H20CLN3O
Molecular Weight	401.894
Exact Mass	401.129
CAS #	174262-13-6
Related CAS #	174262-13-6;
PubChem CID	9952928
Appearance	Off-white to yellow solid powder
Density	1.2±0.1 g/cm3
Boiling Point	651.2±55.0 °C at 760 mmHg
Flash Point	347.7±31.5 °C
Vapour Pressure	0.0±1.9 mmHg at 25°C
Index of Refraction	1.634
LogP	4.54
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	6
Heavy Atom Count	29
Complexity	508
Defined Atom Stereocenter Count	0
InChi Key	FHHNSSGZEIVGMS-UHFFFAOYSA-N
InChi Code	InChI=1S/C24H20ClN3O/c25-22-12-10-19(11-13-22)18-6-8-21(9-7-18)24(29)27-16-23(28-15-14-26-17-28)20-4-2-1-3-5-20/h1-15,17,23H,16H2,(H,27,29)
Chemical Name	N-(2-(1H-imidazol-1-yl)-2-phenylethyl)-4'-chloro-[1,1'-biphenyl]-4-carboxamide
Synonyms	NVP-VID-400 NVP-VID 400 NVP-VID400 SDZ285428 SDZ 285428 SDZ-285428 SDZ285-428 SDZ 285-428 SDZ-285-428.
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vivo	Treatment for human trypanosomiasis may be possible by the selective suppression of protozoal CYP51 [1].
References	[1]. CYP51: A major drug target in the cytochrome P450 superfamily. Lipids. 2008 Dec;43(12):1117-25.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~124.41 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (5.18 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4882 mL	12.4412 mL	24.8824 mL
	5 mM	0.4976 mL	2.4882 mL	4.9765 mL
	10 mM	0.2488 mL	1.2441 mL	2.4882 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.