NUDT5/14 antagonist 1 (Compound 9) is a dual selective antagonist of nucleotide diphosphate kinases NUDT5 and NUDT14 with IC50 of 0.27 and 0.16 μM. NUDT5/14 antagonist 1 binds to Bruton's tyrosine kinase (BTK) with IC50 of 0.377 μM.

Physicochemical Properties

Molecular Formula	C23H24N6O
Molecular Weight	400.48
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: 0.27 μM (NUDT5), 0.16 μM (NUDT14)
ln Vitro	NUDT5/14 antagonist 1 (>10 μM, 72 h) is not cytotoxic in BT-474 cancer cells [1].
Cell Assay	Cell Viability Assay[1] Cell Types: BT-474 Tested Tested Concentrations: >10 μM Incubation Duration: 72 h Experimental Results: Maintained cell viability with Tested Tested Concentrations of 10 μM.
References	[1]. Unexpected Noncovalent Off-Target Activity of Clinical BTK Inhibitors Leads to Discovery of a Dual NUDT5/14 Antagonist. J Med Chem. 2024 May 9;67(9):7245-7259.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4970 mL	12.4850 mL	24.9700 mL
	5 mM	0.4994 mL	2.4970 mL	4.9940 mL
	10 mM	0.2497 mL	1.2485 mL	2.4970 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.