NSP-AS is a chemiluminescent acridine substrate II that can be used for homogeneous analysis.

Physicochemical Properties

Molecular Formula	C28H28N2O8S2
Molecular Weight	584.66
Exact Mass	584.129
CAS #	211106-69-3
PubChem CID	15348931
Appearance	Light yellow to yellow solid powder
LogP	5.381
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	10
Heavy Atom Count	40
Complexity	1060
Defined Atom Stereocenter Count	0
InChi Key	VYTOBATYLKBDAR-UHFFFAOYSA-N
InChi Code	InChI=1S/C28H28N2O8S2/c1-20-13-15-21(16-14-20)40(37,38)30(18-6-12-26(31)32)28(33)27-22-8-2-4-10-24(22)29(17-7-19-39(34,35)36)25-11-5-3-9-23(25)27/h2-5,8-11,13-16H,6-7,12,17-19H2,1H3,(H-,31,32,34,35,36)
Chemical Name	3-[9-[3-carboxypropyl-(4-methylphenyl)sulfonylcarbamoyl]acridin-10-ium-10-yl]propane-1-sulfonate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Novel Applications of Acridinium Compounds and Derivatives in Homogeneous Assays. Patent US2009029349.

Solubility Data

Solubility (In Vitro)

DMSO: 14.29 mg/mL (24.44 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.43 mg/mL (2.45 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.7104 mL	8.5520 mL	17.1040 mL
	5 mM	0.3421 mL	1.7104 mL	3.4208 mL
	10 mM	0.1710 mL	0.8552 mL	1.7104 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.