NSC59984 is a novel and potenl p53 activator with potential anticancer activity. It works by causing the degradation of mutant p53 protein and the activation of p73.

Physicochemical Properties

Molecular Formula	C12H15N3O4
Molecular Weight	265.27
Exact Mass	265.106
Elemental Analysis	C, 54.33; H, 5.70; N, 15.84; O, 24.12
CAS #	803647-40-7
Related CAS #	803647-40-7
PubChem CID	5356520
Appearance	Light yellow to yellow solid powder
Density	1.3±0.1 g/cm3
Boiling Point	451.8±45.0 °C at 760 mmHg
Flash Point	227.1±28.7 °C
Vapour Pressure	0.0±1.1 mmHg at 25°C
Index of Refraction	1.596
LogP	0.82
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	2
Heavy Atom Count	19
Complexity	372
Defined Atom Stereocenter Count	0
SMILES	O=C(N1CCN(C)CC1)C=CC1=CC=C([N+](=O)[O-])O1
InChi Key	QKTRIGNWBRHBFV-DUXPYHPUSA-N
InChi Code	InChI=1S/C12H15N3O4/c1-13-6-8-14(9-7-13)11(16)4-2-10-3-5-12(19-10)15(17)18/h2-5H,6-9H2,1H3/b4-2+
Chemical Name	(E)-1-(4-methylpiperazin-1-yl)-3-(5-nitrofuran-2-yl)prop-2-en-1-one
Synonyms	NSC59984; NSC 59984; NSC-59984
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	p53
ln Vitro	NSC59984 specifically induces p73-dependent cell death and restores p53 pathway signaling in mutant p53-expressing human colorectal cancer cells. Additionally, NSC59984 causes cell death in a panel of cancer cell lines with various p53 mutations while causing little to no genotoxicity in healthy cells. [1]
ln Vivo	NSC59984 (45 mg/kg, i.p.) significantly inhibits tumor growth in nude mice with DLD-1 xenograft tumors in a p73-dependent manner. [1]
Cell Assay	Cells are seeded at 5,000 cells per well on 96-well plates. The manufacturer's recommended procedure is followed when mixing cells with an equal volume of CellTiter-Glo reagents, and the IVIS imager is used to measure the bioluminescence.
Animal Protocol	45 mg/kg N/A Nude mice bearing DLD-1 xenograft tumors
References	[1]. Small-Molecule NSC59984 Restores p53 Pathway Signaling and Antitumor Effects against Colorectal Cancer via p73 Activation and Degradation of Mutant p53. Cancer Res. 2015 Sep 15;75(18):3842-52. [2]. Small-Molecule NSC59984 Restores p53 Pathway Signaling and Antitumor Effects againstColorectal Cancer via p73 Activation and Degradation of Mutant p53. Cancer Res. 2015 Sep 15;75(18):3842-52.

Solubility Data

Solubility (In Vitro)

DMSO: ~53.0 mg/mL (~199.8 mM) Water: <1 mg/mL (slightly soluble or insoluble) Ethanol: ~53.0 mg/mL (~199.8 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.42 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.42 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.7697 mL	18.8487 mL	37.6974 mL
	5 mM	0.7539 mL	3.7697 mL	7.5395 mL
	10 mM	0.3770 mL	1.8849 mL	3.7697 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.