NSC228155 is a novel and potent activator of EGFR, which binds to the sEGFR dimerization domain II and modulate EGFR tyrosine phosphorylation. Previous study found that NSC228155 could dose-dependently inhibit KIX–KID interaction as measured by the split RLuc assay. In living HEK 293T cells, NSC228155 could inhibit CREB-mediated gene transcription with an IC50 of 2.09 μM. NSC228155 also inhibited VP16-CREB-mediated gene transcription with an IC50 of 6.14 μM. Though this was around 3-fold higher than the IC50 of CREB-mediated gene transcription, such results indicated that NSC228155 was not particularly selective in inhibiting KIX–KID interaction inside these living cells.

Physicochemical Properties

Molecular Formula	C11H6N4O4S
Molecular Weight	290.25
Exact Mass	290.01
CAS #	113104-25-9
Related CAS #	113104-25-9
PubChem CID	313619
Appearance	Light yellow to orange solid powder
Density	1.7±0.1 g/cm3
Boiling Point	627.3±65.0 °C at 760 mmHg
Flash Point	333.2±34.3 °C
Vapour Pressure	0.0±1.8 mmHg at 25°C
Index of Refraction	1.793
LogP	0.07
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	2
Heavy Atom Count	20
Complexity	369
Defined Atom Stereocenter Count	0
InChi Key	ICCFXXDUYSPKOL-UHFFFAOYSA-N
InChi Code	InChI=1S/C11H6N4O4S/c16-14-6-2-1-3-9(14)20-8-5-4-7(15(17)18)10-11(8)13-19-12-10/h1-6H
Chemical Name	7-nitro-4-(1-oxidopyridin-1-ium-2-yl)sulfanyl-2,1,3-benzoxadiazole
Synonyms	NSC228155; NSC-228155; NSC 228155
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Through the activity of SOD1, NSC 228155 (100 μM) increases EGFR tyrosine phosphorylation[1]. With IC50 values of 2.09 and 6.14 μM, respectively, NSC 228155 (compound 1) suppresses CREB- and VP16-CREB-mediated gene transcription in vivo HEK 293T cells [2]. In live HEK 293T cells, NSC 228155 is not selective for CREB-mediated gene transcription [2].
ln Vivo	Up to now, there is no animal in vivo data reported.
Animal Protocol
References	[1]. Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1. Sci Rep. 2016 Feb 17;6:21088. [2]. Identification, synthesis and evaluation of substituted benzofurazans as inhibitors of CREB-mediated gene transcription. Bioorg Med Chem Lett. 2013 Oct 1;23(19):5371-5.

Solubility Data

Solubility (In Vitro)

DMSO:58 mg/mL (199.8 mM) Water:<1 mg/mL Ethanol:<1 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: 2 mg/mL (6.89 mM) in 15% Cremophor EL + 85% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.6 mg/mL (5.51 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.4453 mL	17.2265 mL	34.4531 mL
	5 mM	0.6891 mL	3.4453 mL	6.8906 mL
	10 mM	0.3445 mL	1.7227 mL	3.4453 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.