NSC-70220 is a selective allosteric SOS1 inhibitor. NSC-70220 inhibits allosteric site activation and partially inhibits catalytic site activation. NSC-70220 has anti-neoplastic activities.

Physicochemical Properties

Molecular Formula	C22H15NO2
Molecular Weight	325.36
Exact Mass	325.11
CAS #	4551-00-2
PubChem CID	6184791
Appearance	Light yellow to yellow solid powder
Density	1.321g/cm3
Boiling Point	569.6ºC at 760 mmHg
Flash Point	274.9ºC
Index of Refraction	1.767
LogP	6.243
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	2
Heavy Atom Count	25
Complexity	523
Defined Atom Stereocenter Count	0
SMILES	O=[N+]([O-])C1=CC=CC=C1/C=C/C=C2C3=CC=CC=C3C4=CC=CC=C\24
InChi Key	DAVRONXTELQQCO-VQHVLOKHSA-N
InChi Code	InChI=1S/C22H15NO2/c24-23(25)22-15-6-1-8-16(22)9-7-14-21-19-12-4-2-10-17(19)18-11-3-5-13-20(18)21/h1-15H/b9-7+
Chemical Name	9-[(E)-3-(2-nitrophenyl)prop-2-enylidene]fluorene
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product is not stable in solution, please use freshly prepared working solution for optimal results.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	SOS1[1].
ln Vitro	The average OD value in the control group minus the average OD value in the treatment group divided by the average OD value in the control group×100% yields the growth inhibitory rate (IR), which is compared. The growth inhibitory rate of NSC-70220 at 40 μM and NSC-658497 at 30 μM treatments is 29% and 8%, respectively. The growth inhibitory rate of 54% obtained from the combination treatment demonstrates the synergistic effect of NSC-658497 and NSC-70220 on the suppression of cell growth. It was demonstrated that P-AKT and P-ERK expression levels were decreased following treatment with NSC-658497 at 40 μM for two hours either alone or in combination with treatment with 40 μM of NSC-70220 overnight. P-ERK expression levels decreased over night after treatment with 40 μM of NSC-70220[1].
References	[1]. ARYL SULFONOHYDRAZIDES. WO 2016077793A1

Solubility Data

Solubility (In Vitro)

DMSO: 12.5 mg/mL (38.42 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (3.84 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (3.84 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.0735 mL	15.3676 mL	30.7352 mL
	5 mM	0.6147 mL	3.0735 mL	6.1470 mL
	10 mM	0.3074 mL	1.5368 mL	3.0735 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.