Pim-1/2 kinase inhibitor 1 is an orally bioavailable Pim-1/2 kinase inhibitor. Pim-1/2 kinase inhibitor 1 can block the phosphorylation ability of Pim kinase to peptides and inhibit the Pim protein kinase-directed phosphorylation of 4E-BP1 and p27Kip1. Pim-1/2 kinase inhibitor 1 may be utilized in cancer-related research, especially prostate cancer.

Physicochemical Properties

Molecular Formula	C11H9NO3S
Molecular Weight	235.25906
Exact Mass	235.03
CAS #	6320-51-0
PubChem CID	5373936
Appearance	Light yellow to yellow solid powder
Density	1.391g/cm3
Index of Refraction	1.667
LogP	2.347
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	2
Heavy Atom Count	16
Complexity	332
Defined Atom Stereocenter Count	0
Chemical Name	5-[(4-methoxyphenyl)methylidene]thiazolidine-2,4-dione
Synonyms	NSC 31205NSC31205NSC-31205
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Pim-1/2 kinase inhibitor 1 (compound D14) exhibited cytotoxicity against PC3 cells at different concentrations over a 48-hour period, with an IC <50 of 11 µM [1].
Cell Assay	Cytotoxicity assay [1] Cell Types: PC3 cells Tested Concentrations: Different concentrations Incubation Duration: 48 hrs (hours) Experimental Results: Inhibit PC3 cells, IC<50 is 11 µM.
References	[1]. Inhibitors of PIM-1 Protein Kinases, Compositions and Methods for Treating Prostate Cancer. Patent US20110263664A1.

Solubility Data

Solubility (In Vitro)

DMSO : ~250 mg/mL (~1062.65 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (8.84 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.2506 mL	21.2531 mL	42.5062 mL
	5 mM	0.8501 mL	4.2506 mL	8.5012 mL
	10 mM	0.4251 mL	2.1253 mL	4.2506 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.