NPBA is a potassium K2P channel TASK-3 (KCNK9) agonist and a tandem pore-domain weak inwardly rectifier K+ channel (TWIK2) channel blocker. NPBA inhibits NLRP3 inflammasome activation in macrophages.

Physicochemical Properties

Molecular Formula	C16H14F3N3O3
Molecular Weight	353.295874118805
Exact Mass	353.098
CAS #	524033-40-7
PubChem CID	2930995
Appearance	Light yellow to green yellow solid powder
LogP	3.6
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	5
Heavy Atom Count	25
Complexity	462
Defined Atom Stereocenter Count	0
InChi Key	LUJDPMMSYZQCHA-UHFFFAOYSA-N
InChi Code	InChI=1S/C16H14F3N3O3/c17-16(18,19)13-10-12(22(24)25)6-7-14(13)20-8-9-21-15(23)11-4-2-1-3-5-11/h1-7,10,20H,8-9H2,(H,21,23)
Chemical Name	N-[2-[4-nitro-2-(trifluoromethyl)anilino]ethyl]benzamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1].A Small-Molecule Compound Selectively Activates K2P Channel TASK-3 by Acting at Two Distant Clusters of Residues. Mol Pharmacol. 2019 Jul;96(1):26-35. [2].A novel TWIK2 channel inhibitor binds at the bottom of the selectivity filter and protects against LPS-induced experimental endotoxemia in vivo. Biochem Pharmacol. 2023 Dec:218:115894.

Solubility Data

Solubility (In Vitro)

DMSO :~100 mg/mL (~283.05 mM; with sonication)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.08 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 400 μL PEG300 and mix well. Then add 50 μL Tween-80 to the above system and mix well. Then continue to add 450 μL of physiological saline to make up to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.8305 mL	14.1523 mL	28.3046 mL
	5 mM	0.5661 mL	2.8305 mL	5.6609 mL
	10 mM	0.2830 mL	1.4152 mL	2.8305 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.