N-Methylisatoic anhydride (NMIA) is a 2'-OH selective acylating agent of RNA and is extensively used to resolve secondary RNA structures using SHAPE (Selective 2'-OH Acylation by Primer Extension Analysis) technology.

Physicochemical Properties

Molecular Formula	C9H7NO3
Molecular Weight	177.159
Exact Mass	177.043
CAS #	10328-92-4
PubChem CID	25160
Appearance	White to yellow solid powder
Density	1.348 g/cm3
Boiling Point	297.5ºC at 760 mmHg
Melting Point	165 °C (dec.)(lit.)
Flash Point	133.7ºC
Vapour Pressure	0.00135mmHg at 25°C
Index of Refraction	1.583
LogP	0.491
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	0
Heavy Atom Count	13
Complexity	251
Defined Atom Stereocenter Count	0
SMILES	O=C(N(C)C1=CC=CC=C12)OC2=O
InChi Key	KJMRWDHBVCNLTQ-UHFFFAOYSA-N
InChi Code	InChI=1S/C9H7NO3/c1-10-7-5-3-2-4-6(7)8(11)13-9(10)12/h2-5H,1H3
Chemical Name	1-methyl-3,1-benzoxazine-2,4-dione
Synonyms	N-Methylisatoic Anhydride; NMIA
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Additional Infomation

N-methylisatoic anhydride is a 3,1-benzoxazin-1,4-dione having an N-methyl substituent.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~564.46 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (14.11 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.11 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.6446 mL	28.2231 mL	56.4462 mL
	5 mM	1.1289 mL	5.6446 mL	11.2892 mL
	10 mM	0.5645 mL	2.8223 mL	5.6446 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.