NHE3-IN-2 is a Na+/H+ exchanger 3 (NHE3) inhibitor (For more details, check and find patent WO2001079186A1 6-Chlor-4-phenyl-2-chinazolinyl-guanidin).

Physicochemical Properties

Molecular Formula	C15H12CLN5
Molecular Weight	297.74
Exact Mass	297.078
CAS #	92434-13-4
PubChem CID	3145916
Appearance	Light yellow to green yellow solid powder
Density	1.4±0.1 g/cm3
Boiling Point	552.7±42.0 °C at 760 mmHg
Flash Point	288.1±27.9 °C
Vapour Pressure	0.0±1.5 mmHg at 25°C
Index of Refraction	1.725
LogP	2.67
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	2
Heavy Atom Count	21
Complexity	378
Defined Atom Stereocenter Count	0
InChi Key	RGJFEUSQLIFRJG-UHFFFAOYSA-N
InChi Code	InChI=1S/C15H12ClN5/c16-10-6-7-12-11(8-10)13(9-4-2-1-3-5-9)20-15(19-12)21-14(17)18/h1-8H,(H4,17,18,19,20,21)
Chemical Name	2-(6-chloro-4-phenylquinazolin-2-yl)guanidine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. 2-guanidino-4-aryl-chinazoline als nhe-3-inhibitoren. WO2001079186A1.
Additional Infomation	2-(6-chloro-4-phenyl-2-quinazolinyl)guanidine is a member of pyrimidines.

Solubility Data

Solubility (In Vitro)

DMSO: 3.33 mg/mL (11.18 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 0.33 mg/mL (1.11 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 3.3 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.33 mg/mL (1.11 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 3.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.3586 mL	16.7932 mL	33.5864 mL
	5 mM	0.6717 mL	3.3586 mL	6.7173 mL
	10 mM	0.3359 mL	1.6793 mL	3.3586 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.