NDI-Lyso is a lysosome-targeted anticancer agent that forms rigid long fibers in cancer cell lysosomes through the enzyme-directed self-assembly (EISA) mechanism catalyzed by cat ovalbumin B, inducing lysosomal swelling, membrane permeabilization (LMP) and membrane disruption, thereby inducing cancer cell apoptosis through a non-classical caspase-independent pathway. NDI-Lyso exhibits significant selective anticancer activity in a variety of cancer cell lines and drug-resistant cancer cells (IC50 of approximately 10 μM), and has low toxicity to normal cells (IC50 > 60 μM).

Physicochemical Properties

Molecular Formula	C71H100N22O13
Molecular Weight	1469.69
CAS #	2999646-19-2
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Intra-Lysosomal Peptide Assembly for the High Selectivity Index against Cancer. J Am Chem Soc. 2023 Aug 23;145(33):18414-18431.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.6804 mL	3.4021 mL	6.8042 mL
	5 mM	0.1361 mL	0.6804 mL	1.3608 mL
	10 mM	0.0680 mL	0.3402 mL	0.6804 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.