N4-Acetylcytidine triphosphate sodium can be effectively used as a substrate for in vitro transcription catalyzed by T7 RNA polymerase and can be integrated into multiple templates.

Physicochemical Properties

Molecular Formula	C11H14N3NA4O15P3
Molecular Weight	613.120329380035
Exact Mass	612.922
CAS #	2803886-33-9
Related CAS #	N4-Acetylcytidine triphosphate;1428903-57-4
PubChem CID	146673014
Appearance	White to yellow solid powder
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	15
Rotatable Bond Count	9
Heavy Atom Count	36
Complexity	980
Defined Atom Stereocenter Count	4
SMILES	P(=O)([O-])(OP(=O)([O-])OP(=O)([O-])[O-])OC[C@@H]1[C@H]([C@H]([C@H](N2C(N=C(C=C2)NC(C)=O)=O)O1)O)O.[Na+].[Na+].[Na+].[Na+]
InChi Key	VSBLTNBKSQTQJC-XGZYCNACSA-J
InChi Code	InChI=1S/C11H18N3O15P3.4Na/c1-5(15)12-7-2-3-14(11(18)13-7)10-9(17)8(16)6(27-10)4-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,6,8-10,16-17H,4H2,1H3,(H,22,23)(H,24,25)(H2,19,20,21)(H,12,13,15,18);;;;/q;4*+1/p-4/t6-,8-,9-,10-;;;;/m1..../s1
Chemical Name	tetrasodium;[[[(2R,3S,4R,5R)-5-(4-acetamido-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-oxidophosphoryl] phosphate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage.(2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.(3). This product is not stable in solution, please use freshly prepared working solution for optimal results.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	N4-Acetylcytidine triphosphate sodium (0.1-2 mM; 24-48 h) suppresses BV2 microglia development [1]. N4-Acetylcytidine triphosphate sodium (0.3–1 mM; 3–6 h) increases the expression of NLRP3 and initiates NFκB signaling [1]. HMGB1 expression is elevated by N4-acetylcytidine triphosphate sodium (0.3–1 mM; 3 h) [1]. (br>
Cell Assay	Cell Viability Assay[1] Cell Types: BV2 microglia Tested Concentrations: 0.1-2 mM Incubation Duration: 24-48 hrs (hours) Experimental Results: Dramatically inhibited BV2 microglia cell growth util cultured for 48 hrs (hours) with a concentration of 0.3 mM. Cell Viability Assay [1] Cell Types: BV2 microglia Tested Concentrations: 0.3 and 1 mM Incubation Duration: 3 and 6 hrs (hours) Experimental Results: Increased NLRP3 and NFκB expression at both mRNA and protein levels, activated the NFκB signaling. Western Blot Analysis[1] Cell Types: BV2 microglia Tested Concentrations: 0.3 and 1 mM Incubation Duration: 3 hrs (hours) Experimental Results: Induced HMGB1 expression and it is necessary for NFkB signaling, NLRP3 expression and persistent HMGB1 expression.
References	[1]. Profiling Cytidine Acetylation with Specific Affinity and Reactivity. ACS Chem Biol. 2017 Dec 15;12(12):2922-2926.

Solubility Data

Solubility (In Vitro)	H2O :~100 mg/mL (~163.10 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 25 mg/mL (40.78 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with heating and sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.6310 mL	8.1550 mL	16.3100 mL
	5 mM	0.3262 mL	1.6310 mL	3.2620 mL
	10 mM	0.1631 mL	0.8155 mL	1.6310 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.