N-Nitrosonornicotine is a tobacco-specific nitrosamine that is carcinogenic and mutagenic and can induce micronuclei in C3A cells. N-Nitrosonornicotine can form DNA adducts.

Physicochemical Properties

Molecular Formula	C9H11N3O
Molecular Weight	177.20
CAS #	16543-55-8
SMILES	O=N[N]1=CC(=CC=C1)C1CCCN1
Synonyms	(2S)-N'-Nitrosonornicotine
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Metabolism-dependent mutagenicity of two structurally similar tobacco-specific nitrosamines (N-nitrosonornicotine and N-nitrosoanabasine) in human cells, partially different CYPs being activating enzymes. Toxicology. 2024 May:504:153774. [2]. Cotinine, a major nicotine metabolite, induces cell proliferation on urothelium in vitro and in vivo. Toxicology. 2020 Jan 15:429:152325.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.6433 mL	28.2167 mL	56.4334 mL
	5 mM	1.1287 mL	5.6433 mL	11.2867 mL
	10 mM	0.5643 mL	2.8217 mL	5.6433 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.