N-Ethyl-N-nitrosourea (ENU) is a DNA alkylating agent. N-Ethyl-N-nitrosourea induces leukemia by alkylating nucleobases, damaging DNA, leading to bone marrow suppression and the formation of leukemic cells. N-Ethyl-N-nitrosourea is teratogenic in vivo, inducing tumor formation and foot deformities in pregnant rats. N-Ethyl-N-nitrosourea causes central nervous system (CNS) tumors and hereditary diseases.

Physicochemical Properties

Molecular Formula	C3H7N3O2
Molecular Weight	117.11
CAS #	759-73-9
SMILES	CCN(N=O)C(N)=O
Synonyms	ENU; N-Nitroso-N-ethylurea
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. N-Ethyl-N-Nitrosourea Induced Leukaemia in a Mouse Model: Protective Effect of Icaritin via Inhibition of IL-6/JAK2/STAT3 Pathway Causes Apoptosis. J Inflamm Res. 2024 Feb 7;17:777-790. [2]. Persistence of O6-ethylguanine in rat-brain DNA: correlation with nervous system-specific carcinogenesis by ethylnitrosourea. Proc Natl Acad Sci U S A. 1974 Mar;71(3):639-43.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	8.5390 mL	42.6949 mL	85.3898 mL
	5 mM	1.7078 mL	8.5390 mL	17.0780 mL
	10 mM	0.8539 mL	4.2695 mL	8.5390 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.