Myrothecine A is a trichothecene toxin found in M. roridum. Myrothecine A induces apoptosis, promotes cytochrome c release, PARP cleavage and JNK phosphorylation, increases Bax and cleaved caspase-3, -5 and -8 levels. Myrothecine A has anticancer activity and promotes maturation of dendritic cells in the microenvironment. Myrothecine A inhibits the proliferation of A549, MCF-7, HepG2 and SMMC-7721 cancer cells with IC50 values of 95, 70, 60 and 25 µM, respectively.

Physicochemical Properties

Molecular Formula	C29H38O10
Molecular Weight	546.61
CAS #	910292-40-9
SMILES	O1[C@@H]2C[C@@H]3[C@@]4(C)[C@@]2(C[C@@H]2[C@@](C)(CC[C@@]4(COC(C=C4CCO[C@@](C(C)=O)(CCC=CC(=O)O3)[C@@H]4O)=O)[C@H]12)O)O |c:30|
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets

Caspase 3 Caspase-5 Caspase-8 Bax

References

[1]. Myrothecine A modulates the proliferation of HCC cells and the maturation of dendritic cells through downregulating miR-221. Int Immunopharmacol. 2019 Oct;75:105783. [2]. Structure elucidation and biological activity of two new trichothecenes from an endophyte, Myrothecium roridum. J Agric Food Chem. 2014 Jun 25;62(25):5993-6000. [3]. Mytoxin B and Myrothecine A Induce Apoptosis in Human Hepatocarcinoma Cell Line SMMC-7721 via PI3K/Akt Signaling Pathway. Molecules. 2019 Jun 20;24(12):2291.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.8295 mL	9.1473 mL	18.2946 mL
	5 mM	0.3659 mL	1.8295 mL	3.6589 mL
	10 mM	0.1829 mL	0.9147 mL	1.8295 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.