Micronomicin sulfate (Gentamicin C2b sulfate) is an aminoglycoside antibiotic extracted from Micromonospora. Micronomicin sulfate is a broad-spectrum antibiotic close to gentamicin antibiotics. It has high inhibitory effect against Pseudomonas, Proteus, Klebsiella pneumoniae, Serratia, etc. (MIC=0.001-8.3 μg/ml ).

Physicochemical Properties

Molecular Formula	C20H41N5O7.XH2SO4
Molecular Weight	561.64732
Exact Mass	561.268
CAS #	66803-19-8
Related CAS #	Micronomicin;52093-21-7
PubChem CID	171808
Appearance	White to yellow solid powder
Density	1.32g/cm3
Boiling Point	667.2ºC at 760mmHg
Flash Point	357.3ºC
Hydrogen Bond Donor Count	10
Hydrogen Bond Acceptor Count	16
Rotatable Bond Count	7
Heavy Atom Count	37
Complexity	688
Defined Atom Stereocenter Count	12
SMILES	C[C@@]1(CO[C@@H]([C@@H]([C@H]1NC)O)O[C@H]2[C@@H](C[C@@H]([C@H]([C@@H]2O)O[C@@H]3[C@@H](CC[C@H](O3)CNC)N)N)N)O.OS(=O)(=O)O
InChi Key	MQDGQSCLOYLSEK-SCFBDNQUSA-N
InChi Code	InChI=1S/C20H41N5O7.H2O4S/c1-20(28)8-29-19(14(27)17(20)25-3)32-16-12(23)6-11(22)15(13(16)26)31-18-10(21)5-4-9(30-18)7-24-2;1-5(2,3)4/h9-19,24-28H,4-8,21-23H2,1-3H3;(H2,1,2,3,4)/t9-,10+,11-,12+,13-,14+,15+,16-,17+,18+,19+,20-;/m0./s1
Chemical Name	(2R,3R,4R,5R)-2-[(1S,2S,3R,4S,6R)-4,6-diamino-3-[(2R,3R,6S)-3-amino-6-(methylaminomethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Aminoglycoside
ln Vitro	Micronomicin exhibits strong antibacterial properties; it effectively combats Staphylococcus aureus FDA 209 P, exhibiting minimal inhibitory values of 0.01 μg/ml. Additionally, it is anti-Escherichia coli St. M. The minimal inhibitory values were 0.75 μg/ml, 0.3 μg/ml, 0.03 μg/ml, and 0.03 μg/ml for 589, Baker 2, F 14-BK, and R5/W677. Additionally, it exhibits activity against strains of Lebsiella pneumoniae and Pseudomonas aeruginosa (MICs = 0.03-17.5 μg/ml)[1].
ln Vivo	Micronomicin sulfate has an intravenous acute LD50 of 93 mg/kg in mice and is very effective against a variety of bacterial infections in mice[1]. Subacute toxicity studies involve the intravenous injection of micronomicin sulfate at a dose of 4-100 mg/kg over a 30-day period. Ataxia and renal problems cause the wistar rats (10 out of 30 animals) to die at the 100 mg/kg dose. Renal histopathological abnormalities primarily manifest at dosages beyond 25 mg/kg [3].
Animal Protocol	Animal/Disease Models: Wistar rats[3] Doses: 4, 10, 25, 63 mg/kg and 100 mg/kg Route of Administration: intravenous (iv)injection; 30 days Experimental Results: Led to death of rat at 100 mg/kg.
References	[1]. A New Antibiotic XK-62-2 (Sagamicin). I. Isolation, Physicochemical and Antibacterial Properties. J Antibiot (Tokyo). [2]. P J Daniels, The Gentamicin Antibiotics. 6. Gentamicin C2b, an Aminoglycoside Antibiotic Produced by Micromonospora Purpurea Mutant JI-33. J Antibiot (Tokyo). [3]. Safety Evaluation of Micronomicin V. Subacute Toxicity in Rats After Intravenous Injection. Jpn J Antibiot. 1983 Nov;36(11):3208-25.
Additional Infomation	Micronomicin sulfate is an aminoglycoside sulfate salt. It is functionally related to a micronomicin.

Solubility Data

Solubility (In Vitro)	H2O : 50 mg/mL
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.7805 mL	8.9023 mL	17.8047 mL
	5 mM	0.3561 mL	1.7805 mL	3.5609 mL
	10 mM	0.1780 mL	0.8902 mL	1.7805 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.