Methyl nonadecanoate serves as an internal standard for the determination of fatty acid methyl ester (FAME) content in biodiesel.

Physicochemical Properties

Molecular Formula	C20H40O2
Molecular Weight	312.5304
Exact Mass	312.302
CAS #	1731-94-8
PubChem CID	15610
Appearance	White to off-white solid powder
Density	0.9±0.1 g/cm3
Boiling Point	362.8±5.0 °C at 760 mmHg
Melting Point	37-40 °C(lit.)
Flash Point	176.9±7.5 °C
Vapour Pressure	0.0±0.8 mmHg at 25°C
Index of Refraction	1.446
LogP	9.21
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	18
Heavy Atom Count	22
Complexity	226
Defined Atom Stereocenter Count	0
InChi Key	BDXAHSJUDUZLDU-UHFFFAOYSA-N
InChi Code	InChI=1S/C20H40O2/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20(21)22-2/h3-19H2,1-2H3
Chemical Name	methyl nonadecanoate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Development and validation of analytical methodology by GC-FID using hexadecyl propanoate as an internal standard to determine the bovine tallow methyl esters content.J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Sep 1;1093-1094:13.
Additional Infomation	Methyl nonadecanoate is a fatty acid methyl ester resulting from the formal condensation of the carboxy group of nonadecanoic acid with methanol. It is functionally related to a nonadecanoic acid.

Solubility Data

Solubility (In Vitro)

DMSO : ~10 mg/mL (~32.00 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1 mg/mL (3.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1 mg/mL (3.20 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1 mg/mL (3.20 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.1997 mL	15.9985 mL	31.9969 mL
	5 mM	0.6399 mL	3.1997 mL	6.3994 mL
	10 mM	0.3200 mL	1.5998 mL	3.1997 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.