Melezitose monohydrate (d-Melezitosedihydrate) is a biochemical reagent that can be used as a biomaterial or organic compound related to life science research.

Physicochemical Properties

Molecular Formula	C18H34O17
Molecular Weight	522.45
CAS #	10030-67-8
PubChem CID	16217716
Appearance	White to off-white solid powder
Boiling Point	937.8ºC at 760mmHg
Melting Point	160 °C (dec.)(lit.)
Flash Point	520.9ºC
Vapour Pressure	0mmHg at 25°C
LogP	0
Hydrogen Bond Donor Count	12
Hydrogen Bond Acceptor Count	17
Rotatable Bond Count	8
Heavy Atom Count	35
Complexity	655
Defined Atom Stereocenter Count	14
SMILES	O1[C@H](CO)[C@H]([C@@H]([C@]1(CO)O[C@@H]1[C@@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)O)O[C@@H]1[C@@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)O)O.O
Synonyms	Melezitose monohydrate; d-Melezitosedihydrate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Biochemical reagentsM//Methods of Enzymatic Analysis. Academic Press, 1965: 967-1037.

Solubility Data

Solubility (In Vitro)

DMSO : ~65 mg/mL (~124.41 mM; with ultrasonication)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.17 mg/mL (4.15 mM)(saturation unknown) in 10% DMSO 40% PEG300 5% Tween-80 45% Saline (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution and add it to 400 μL PEG300, mix well; then add 50 μL Tween-80 to the above system, mix well; then continue to add 450 μL of normal saline to make up to 1 mL. Preparation of normal saline: Dissolve 0.9 g of sodium chloride in ddH₂O and make up to 100 mL to obtain a clear and transparent normal saline solution. Solubility in Formulation 2: ≥ 2.17 mg/mL (4.15 mM)(saturation unknown) in 10% DMSO 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution and add it to 900 μL of 20% SBE-β-CD saline solution and mix well. 2 g SBE-β-CD (sulfobutyl ether β-cyclodextrin) powder is diluted to 10 mL of saline and completely dissolved until clear and transparent. Solubility in Formulation 3: ≥ 2.17 mg/mL (4.15 mM)(saturation unknown) in 10% DMSO 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution and add it to 900 μL corn oil and mix well.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9141 mL	9.5703 mL	19.1406 mL
	5 mM	0.3828 mL	1.9141 mL	3.8281 mL
	10 mM	0.1914 mL	0.9570 mL	1.9141 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.