Mal-PEG3-NHS ester is a non-cleavable (non-degradable) ADC linker containing a Maleimide group, may be utilized to prepare antibody-conjugated active molecules.

Physicochemical Properties

Molecular Formula	C17H22N2O9
Molecular Weight	398.364585399628
Exact Mass	398.132
CAS #	1537892-36-6
PubChem CID	77078335
Appearance	Colorless to light yellow liquid
Density	1.4±0.1 g/cm3
Boiling Point	552.4±60.0 °C at 760 mmHg
Flash Point	287.9±32.9 °C
Vapour Pressure	0.0±1.5 mmHg at 25°C
Index of Refraction	1.555
LogP	-2.61
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	9
Rotatable Bond Count	14
Heavy Atom Count	28
Complexity	613
Defined Atom Stereocenter Count	0
SMILES	O(CCOCCOCCC(=O)ON1C(CCC1=O)=O)CCN1C(C=CC1=O)=O
InChi Key	IIXXEVQHRSMNOB-UHFFFAOYSA-N
InChi Code	InChI=1S/C17H22N2O9/c20-13-1-2-14(21)18(13)6-8-26-10-12-27-11-9-25-7-5-17(24)28-19-15(22)3-4-16(19)23/h1-2H,3-12H2
Chemical Name	(2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[2-(2,5-dioxopyrrol-1-yl)ethoxy]ethoxy]ethoxy]propanoate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage.(2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Thiol-bearing ligands can be coupled to amine-functionalized matrices using the heterobifunctional cross-linker maleimide-PEG-NHS ester [1].
References	[1]. Immobilization of hydrophobic peptidic ligands to hydrophilic chromatographic matrix: a preconcentration approach. Anal Biochem. 2012 Apr 15;423(2):202-9.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~251.03 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.28 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.5103 mL	12.5515 mL	25.1029 mL
	5 mM	0.5021 mL	2.5103 mL	5.0206 mL
	10 mM	0.2510 mL	1.2551 mL	2.5103 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.