MS15203 is a potent and specific GPR171 agonist. MS15203 increases food intake and body weight. MS15203 increases neuronal activity. MS15203 significantly increased the abundance of mRNA encoding proSAAS, NPY, and AgRP.

Physicochemical Properties

Molecular Formula	C12H11NO5
Molecular Weight	249.22
Exact Mass	249.064
CAS #	73912-52-4
PubChem CID	773544
Appearance	White to off-white solid powder
Density	1.42g/cm3
Boiling Point	561.3ºC at 760 mmHg
Flash Point	293.2ºC
Index of Refraction	1.639
LogP	1.67
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	4
Heavy Atom Count	18
Complexity	368
Defined Atom Stereocenter Count	0
InChi Key	NVOBVSWSDYFEMR-UHFFFAOYSA-N
InChi Code	InChI=1S/C12H11NO5/c1-6(2)10(14)13-9-4-7(11(15)16)3-8(5-9)12(17)18/h3-5H,1H2,2H3,(H,13,14)(H,15,16)(H,17,18)
Chemical Name	5-(2-methylprop-2-enoylamino)benzene-1,3-dicarboxylic acid
Synonyms	MS 0015203; MS-0015203; MS0015203
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In rat hypothalamus membranes, MS15203 (MS0015203) decreases adenylyl cyclase activity and dose-dependently enhances [[35]S]GTPγS binding [1].
ln Vivo	GPR171-containing cells in the PVN exhibit enhanced neuronal activity in response to MS15203 (MS0015203) (3 mg/kg; i.p.) [1]. ..At 4 and 8 hours, MS15203 (3 mg/kg; i.p.) dramatically improves meal intake [1]. The intraperitoneal injection of MS15203 (3 mg/kg) dramatically raises the quantity of mRNA encoding proSAAS, NPY, and AgRP [1].
References	[1]. Identification of a small-molecule ligand that activates the neuropeptide receptor GPR171 and increases food intake. Sci Signal. 2016 May 31;9(430):ra55.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~200.63 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (10.03 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.0125 mL	20.0626 mL	40.1252 mL
	5 mM	0.8025 mL	4.0125 mL	8.0250 mL
	10 mM	0.4013 mL	2.0063 mL	4.0125 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.