MS-1020 is a potent ATP-competitive JAK3 inhibitor. MS-1020 inhibits JAK3/STAT signaling and causes apoptosis. MS-1020 promotes cell death. MS-1020 reduces the expression level of tyrosine phosphorylated STAT3. MS-1020 may be used for studying cancers harboring aberrant JAK3 signaling.

Physicochemical Properties

Molecular Formula	C21H18N2O3
Molecular Weight	346.386
Exact Mass	346.131
CAS #	1255516-86-9
PubChem CID	73438566
Appearance	Typically exists as solid at room temperature
Density	1.4±0.1 g/cm3
Boiling Point	711.2±60.0 °C at 760 mmHg
Flash Point	383.9±32.9 °C
Vapour Pressure	0.0±2.4 mmHg at 25°C
Index of Refraction	1.758
LogP	3.2
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	4
Heavy Atom Count	26
Complexity	500
Defined Atom Stereocenter Count	0
SMILES	O([H])C1C([H])=C([H])C2=C(C=1[H])C(=C([H])N2[H])C([H])([H])C([H])([H])N([H])C(C1C([H])=C([H])C2=C([H])C([H])=C([H])C([H])=C2C=1O[H])=O
InChi Key	KHJNISGKGIHTMY-UHFFFAOYSA-N
InChi Code	InChI=1S/C21H18N2O3/c24-15-6-8-19-18(11-15)14(12-23-19)9-10-22-21(26)17-7-5-13-3-1-2-4-16(13)20(17)25/h1-8,11-12,23-25H,9-10H2,(H,22,26)
Chemical Name	1-hydroxy-N-[2-(5-hydroxy-1H-indol-3-yl)ethyl]naphthalene-2-carboxamide
Synonyms	MS1020 MS 1020 MS-1020
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In cultured Drosophila cells, MS-1020 (0-50 µM; 24 hours) suppresses the transcriptional activity of STAT92E [1]. While MS-1020 (0, 10, 30, and 50 µM) did not alter total STAT3 levels, it does decrease the expression of tyrosine-phosphorylated STAT3 levels [1]. In Nb2 cells, the expression of IL-2 (100 ng/ml)-induced STAT5 phosphorylation is reduced by MS-1020 (0, 10, 30, 50 µM; 16 hours) [1]. L540 cell death is promoted by MS-1020 in a time- and dose-dependent manner (0, 10, 30, 50 µM; 72 hours) [1]. In L540 cells, MS-1020 (0, 10, 30, 50 µM; 72 hours) causes apoptosis [1].
Cell Assay	Western Blot Analysis[1] Cell Types: L540, HDLM-2, MDA-MB-468 Cell Tested Concentrations: 0, 10, 30, 50 µM Incubation Duration: Experimental Results: diminished levels of tyrosine-phosphorylated STAT3 in a dose-dependent manner expression and no change in total STAT3 levels. Cell viability assay[1] Cell Types: L540, HDLM-2 Cell Tested Concentrations: 0, 10, 30, 50 µM Incubation Duration: 72 hrs (hours) Experimental Results: Promoted cell death in a time- and dose-dependent manner only in L540 cells. Apoptosis analysis[1] Cell Types: L540 Cell Tested Concentrations: 0, 10, 30, 50 µM Incubation Duration: 72 hrs (hours) Experimental Results: Induction of apoptosis in L540 cells and increased poly(ADP-ribose) polymerase (PARP) and cleavage enzymes The expression of cleaved caspase-3 and cleaved caspase-3 diminished the expression of Bcl-2, Bcl-xL, Mcl-1 and survivin in a dose-dependent manner.
References	[1]. MS-1020 is a novel small molecule that selectively inhibits JAK3 activity. Br J Haematol. 2010 Jan;148(1):132-43.

Solubility Data

Solubility (In Vitro)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.8869 mL	14.4346 mL	28.8692 mL
	5 mM	0.5774 mL	2.8869 mL	5.7738 mL
	10 mM	0.2887 mL	1.4435 mL	2.8869 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.