MN-25 (compound 4a) is an orally active indolpyridone that serves as a novel cannabinoid ligand. MN-25 has Ki of 245 nM and 11 nM for CB1 and CB2. MN-25 has CB2 agonist activity to inhibit TNF-R release in human peripheral blood mononuclear cells in vitro with an IC50 of 33 μM. MN-25 failed to show efficacy in a mouse acute inflammation model at oral doses up to 50 mg/kg.

Physicochemical Properties

Molecular Formula	C26H37N3O3
Molecular Weight	439.59
CAS #	501926-82-5
SMILES	CC1([C@@H]2CC[C@@](C2)([C@@H]1NC(C3=CN(C4=C3C=CC=C4OC)CCN5CCOCC5)=O)C)C
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	CB1 254 nM (Ki); CB2 11 nM (Ki)
References	[1]. Rational design and synthesis of an orally active indolopyridone as a novel conformationally constrained cannabinoid ligand possessing antiinflammatory properties. J Med Chem. 2003 May 22;46(11):2110-6.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.2748 mL	11.3742 mL	22.7485 mL
	5 mM	0.4550 mL	2.2748 mL	4.5497 mL
	10 mM	0.2275 mL	1.1374 mL	2.2748 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.