MK6-83 is a candidate agonist of TRPML1 with superior activity. MK6-83 has potential usefulness in type IV mucolipidosis research.

Physicochemical Properties

Molecular Formula	C16H20N2O2S2
Molecular Weight	336.46
Exact Mass	336.096
CAS #	1062271-24-2
PubChem CID	18191179
Appearance	Light yellow to yellow solid powder
Density	1.3±0.1 g/cm3
Boiling Point	502.6±60.0 °C at 760 mmHg
Flash Point	257.8±32.9 °C
Vapour Pressure	0.0±1.3 mmHg at 25°C
Index of Refraction	1.633
LogP	3.4
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	4
Heavy Atom Count	22
Complexity	457
Defined Atom Stereocenter Count	0
InChi Key	IRGYSXZCDAWOOC-UHFFFAOYSA-N
InChi Code	InChI=1S/C16H20N2O2S2/c1-13-9-10-16(21-13)22(19,20)17-14-7-3-4-8-15(14)18-11-5-2-6-12-18/h3-4,7-10,17H,2,5-6,11-12H2,1H3
Chemical Name	5-methyl-N-(2-piperidin-1-ylphenyl)thiophene-2-sulfonamide
Synonyms	MK683; MK6 83; MK6-83
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	MK6-83 does not show enough cytotoxicity between 0.2 and 30 μM [1]. When it comes to fibroblast lysosomes isolated from cells expressing R403C or V446L, MK6-83 seems to be much more powerful than when it comes to cells isolated from TRPML1 −/− cells [1].
Cell Assay	Cell viability assay[1]. Cell Types: Lysosomes isolated from fibroblast cell lines of MLIV patients carrying F408D, R403C or V446L mutations[1]. Tested Concentrations: 0-10μM. Incubation Duration: 24 hrs (hours). Experimental Results: Effective on fibroblast lysosomes isolated from R403C or V446L expressing cells. There was no significant effect on lysosomes isolated from TRPML1-/- fibroblasts.
References	[1]. A Small Molecule Restores Function to TRPML1 Mutant Isoforms Responsible for Mucolipidosis Type IV. Nat Commun. 2014 Aug 14;5:4681.

Solubility Data

Solubility (In Vitro)

DMSO : ~25 mg/mL (~74.30 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.43 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (7.43 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9721 mL	14.8606 mL	29.7212 mL
	5 mM	0.5944 mL	2.9721 mL	5.9442 mL
	10 mM	0.2972 mL	1.4861 mL	2.9721 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.