MHY-1685 is a novel mTOR inhibitor that offers the opportunity to improve hCSC-based myocardial regeneration.

Physicochemical Properties

Molecular Formula	C11H8N2O4
Molecular Weight	232.19
Exact Mass	232.048
CAS #	27406-31-1
PubChem CID	5292673
Appearance	Light yellow to yellow solid powder
LogP	0.799
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Heavy Atom Count	17
Complexity	372
Defined Atom Stereocenter Count	0
InChi Key	KSOJQSBHVKAHPC-UHFFFAOYSA-N
InChi Code	InChI=1S/C11H8N2O4/c14-7-3-1-6(2-4-7)5-8-9(15)12-11(17)13-10(8)16/h1-5,14H,(H2,12,13,15,16,17)
Chemical Name	5-[(4-hydroxyphenyl)methylidene]-1,3-diazinane-2,4,6-trione
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Human cardiac stem cells rejuvenated by modulating autophagy with MHY-1685 enhance the therapeutic potential for cardiac repair. Exp Mol Med. 2021 Sep;53(9):1423-1436.

Solubility Data

Solubility (In Vitro)

DMSO: 62.5 mg/mL (269.18 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.08 mg/mL (8.96 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.3068 mL	21.5341 mL	43.0682 mL
	5 mM	0.8614 mL	4.3068 mL	8.6136 mL
	10 mM	0.4307 mL	2.1534 mL	4.3068 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.