MES (2-Morpholinoethanesulphonic acid) sodium salt is a buffering agent in biology and biochemistry. As a Good's buffer, MES sodium salt has a buffering capacity of pH 5.5-7.0 and can be extensively used to adjust the pH value of plant culture media, reagent solutions and physiological experiments.

Physicochemical Properties

Molecular Formula	C6H12NNAO4S
Molecular Weight	217.22
Exact Mass	217.038
CAS #	71119-23-8
Related CAS #	MES;4432-31-9;MES monohydrate;145224-94-8;MES potassium;39946-25-3;MES-d13;352534-94-2
PubChem CID	23673676
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	13
Complexity	219
Defined Atom Stereocenter Count	0
SMILES	S(C([H])([H])C([H])([H])N1C([H])([H])C([H])([H])OC([H])([H])C1([H])[H])(=O)(=O)[O-].[Na+]
InChi Key	IRHWMYKYLWNHTL-UHFFFAOYSA-M
InChi Code	InChI=1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1
Chemical Name	sodium;2-morpholin-4-ylethanesulfonate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. N E Good, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. [2]. Tomoko Kagenishi, et al. MES Buffer Affects Arabidopsis Root Apex Zonation and Root Growth by Suppressing Superoxide Generation in Root Apex. Front Plant Sci. 2016 Feb 18;7:79.
Additional Infomation	Sodium 2-(N-morpholino)ethanesulfonate is the organic sodium salt that is the sodium salt of 2-(N-morpholino)ethanesulfonic acid. It contains a 2-(N-morpholino)ethanesulfonate.

Solubility Data

Solubility (In Vitro)

DMSO: 25 mg/mL (115.09 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (11.51 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.51 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (11.51 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.6036 mL	23.0181 mL	46.0363 mL
	5 mM	0.9207 mL	4.6036 mL	9.2073 mL
	10 mM	0.4604 mL	2.3018 mL	4.6036 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.