MDL 72527 di-HCl is a potent polyamine oxidase (PAO) inhibitor. MDL 72527 di-HCl displays lysosomal effects. MDL 72527 di-HCl is neuro-protection.

Physicochemical Properties

Molecular Formula	C12H22CL2N2
Molecular Weight	265.22
Exact Mass	264.116
CAS #	93565-01-6
PubChem CID	3035046
Appearance	White to off-white solid powder
Boiling Point	300.7ºC at 760mmHg
Flash Point	159.2ºC
Index of Refraction	1.536
LogP	4.013
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	9
Heavy Atom Count	16
Complexity	184
Defined Atom Stereocenter Count	0
SMILES	Cl.C(=CCNCCCCNCC=C=C)=C
InChi Key	ITVRWVVFVHINOH-UHFFFAOYSA-N
InChi Code	InChI=1S/C12H20N2.2ClH/c1-3-5-9-13-11-7-8-12-14-10-6-4-2;;/h5-6,13-14H,1-2,7-12H2;2*1H
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	polyamine oxidase (PAO)[1]
ln Vitro	MDL 72527 dihydrochloride (300 µM; 24 h) significantly altered the morphology of M14 WT cells, resulting in the formation of several lysosomes and cytoplasmic vacuoles [2].
ln Vivo	Neuroprotective properties of MDL 72527 dihydrochloride (100 mg/kg; ip; once) on neuronal cell death following transient localized cerebral ischemia[1].
Cell Assay	Cell Cytotoxicity Assay[2] Cell Types: M14 cells Tested Concentrations: 300 µM Incubation Duration: 24 h Experimental Results: demonstrated no significant effect on cell survival of melanoma cells.
Animal Protocol	Animal/Disease Models: 250-300 g, Male spontaneously hypertensive rats (SHRs)[1] Doses: 100 mg/kg Route of Administration: Ip; after the induction of ischemia Experimental Results: Dramatically decreased meanarterial blood pressure, changed the brain edema formation from 85.7 ± 0.3 to 84.5 ± 0.9% in cortex and from 79.9 ± 1.7 to 78.4 ± 2.0% in subcortex (difference not significant), decreased ischemic injury volume by 22% in the cortex and 17% in the subcortex.
References	[1]. Effects of MDL 72527, a specific inhibitor of polyamine oxidase, on brain edema, ischemic injury volume, and tissue polyamine levels in rats after temporary middle cerebral artery occlusion. J Neurochem. 1999 Feb;72(2):765-70. [2]. Toxicity of enzymatic oxidation products of spermine to human melanoma cells (M14): sensitization by heat and MDL 72527. Biochim Biophys Acta. 2006 Oct;1763(10):1040-50.

Solubility Data

Solubility (In Vitro)

H2O: 100 mg/mL (377.05 mM) DMSO: 12.5 mg/mL (47.13 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (4.71 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (4.71 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 3: 50 mg/mL (188.52 mM) in Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.7705 mL	18.8523 mL	37.7045 mL
	5 mM	0.7541 mL	3.7705 mL	7.5409 mL
	10 mM	0.3770 mL	1.8852 mL	3.7705 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.