MCP110 is an inhibitor (blocker/antagonist) of Ras/Raf-1 interaction. MCP110 prevents Ras from interacting with Raf. MCP110 may be utilized in human tumor research.

Physicochemical Properties

Molecular Formula	C33H36N2O3
Molecular Weight	508.66
Exact Mass	508.272
CAS #	521310-51-0
PubChem CID	10229237
Appearance	Colorless to light yellow ointment
Density	1.1±0.1 g/cm3
Boiling Point	686.3±55.0 °C at 760 mmHg
Flash Point	368.8±31.5 °C
Vapour Pressure	0.0±2.1 mmHg at 25°C
Index of Refraction	1.594
LogP	6.24
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	14
Heavy Atom Count	38
Complexity	646
Defined Atom Stereocenter Count	0
SMILES	COC1=C(C=CC(=C1)CN(CCC2=CC=CC=N2)C(=O)CCCCC3=CC=CC=C3)OCC4=CC=CC=C4
InChi Key	JWLZIHLAMJDONF-UHFFFAOYSA-N
InChi Code	InChI=1S/C33H36N2O3/c1-37-32-24-29(19-20-31(32)38-26-28-15-6-3-7-16-28)25-35(23-21-30-17-10-11-22-34-30)33(36)18-9-8-14-27-12-4-2-5-13-27/h2-7,10-13,15-17,19-20,22,24H,8-9,14,18,21,23,25-26H2,1H3
Chemical Name	N-[(3-methoxy-4-phenylmethoxyphenyl)methyl]-5-phenyl-N-(2-pyridin-2-ylethyl)pentanamide
Synonyms	MCP 110 MCP-110 MCP110
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Ras and RAF
ln Vitro	In HEK293 cells, MCP110 (10 and 20 μM) suppresses the activation of AP-1 triggered by H-Ras (V12). The activation of Raf-1 activity by Ras is greatly reduced when MCP110 (20 μM) is present [1]. In fibrosarcoma HT1080 cells, MCP110 (1, 2, 5, 10 and 20 μM) shows dose-dependent suppression of increased Raf-1 activity [1]. Growth factors caused by serum, phorbol 12-myristate 13-acetate (100 nM), platelet-derived growth factor in A549 cells (20 ng/mL), and EGF (100 ng/mL) are all reduced by MCP110 (20 μM)[1].
References	[1]. Inhibitors of Ras/Raf-1 Interaction Identified by Two-Hybrid Screening Revert Ras-dependent Transformation Phenotypes in Human Cancer Cells. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14398-403.

Solubility Data

Solubility (In Vitro)

DMSO : ~250 mg/mL (~491.50 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (4.09 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.09 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9659 mL	9.8297 mL	19.6595 mL
	5 mM	0.3932 mL	1.9659 mL	3.9319 mL
	10 mM	0.1966 mL	0.9830 mL	1.9659 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.