MCA is an analogue of coumarin. MCA quantified platelet activating factor using high-performance liquid chromatography fluorescence detection. MCA can modify FRET peptide substrates for analysis of protease activity.

Physicochemical Properties

Molecular Formula	C12H10O5
Molecular Weight	234.20
Exact Mass	234.052
CAS #	62935-72-2
Related CAS #	(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d3;(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6
PubChem CID	342221
Appearance	White to off-white solid powder
Density	1.4±0.1 g/cm3
Boiling Point	472.9±45.0 °C at 760 mmHg
Melting Point	193 °C (dec.)(lit.)
Flash Point	189.2±22.2 °C
Vapour Pressure	0.0±1.2 mmHg at 25°C
Index of Refraction	1.584
LogP	1.52
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	17
Complexity	360
Defined Atom Stereocenter Count	0
InChi Key	ZEKAXIFHLIITGV-UHFFFAOYSA-N
InChi Code	InChI=1S/C12H10O5/c1-16-8-2-3-9-7(4-11(13)14)5-12(15)17-10(9)6-8/h2-3,5-6H,4H2,1H3,(H,13,14)
Chemical Name	2-(7-methoxy-2-oxochromen-4-yl)acetic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. NSP4 is stored in azurophil granules and released by activated neutrophils as active endoprotease with restricted specificity. J Immunol. 2013 Sep 1;191(5):2700-7. [2]. Effect of functional groups on the solubilities of coumarin derivatives in supercritical carbon dioxide. Chromatographia. 1998 Jan;47(93-7).
Additional Infomation	7-methoxycoumarin-4-acetic acid is a monocarboxylic acid. It has a role as a fluorochrome. It is functionally related to a coumarin.

Solubility Data

Solubility (In Vitro)

DMSO: 25 mg/mL (106.75 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (10.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.67 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.2699 mL	21.3493 mL	42.6985 mL
	5 mM	0.8540 mL	4.2699 mL	8.5397 mL
	10 mM	0.4270 mL	2.1349 mL	4.2699 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.