MB-0223 is a potent and selective partial inhibitor of dynamin-related GTPase Drp1 (IC50=1.3 μM), with superior selectivity over other dynamin family members, Opa1 and dynamin-1 (IC50>100 μM).

Physicochemical Properties

Molecular Formula	C26H27N5OS
Molecular Weight	457.59
CAS #	2247732-89-2
SMILES	S1C=C(CN2C=C(C)C=C2C(NC2C=CC(C3C=NC=CC=3)=CC=2)=O)N=C1N1CCCCC1
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: 1.3 μM (Drp1)[1]
ln Vitro	MB-0223 (30 μM; 48 hours) increases mitochondrial DNA levels in mfn1- knockout MEFs deficient in mitochondrial fusion[1]. MB-0223 (0-100 μM) is a partial inhibitor and exhibits a maximum inhibition of 67% of Drp1 activity. MB-0223 exhibits >100-fold selectivity for Drp1 over two other dynamin-like GTPases, Opa1 and Dnm1[1].
References	[1]. Characterization of Small Molecule Inhibitors of Drp1.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1854 mL	10.9268 mL	21.8536 mL
	5 mM	0.4371 mL	2.1854 mL	4.3707 mL
	10 mM	0.2185 mL	1.0927 mL	2.1854 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.