MAC-5576 is a SARS-CoV-2 3CL protease inhibitor with IC50 of 81 nM. MAC-5576 inhibits 3CLpro (IC50 = 0.5 μM), HAV 3Cpro (IC50 = 0.5 μM), and chymotrypsin (IC50 = 13 μM).

Physicochemical Properties

Molecular Formula	C10H6CLNO2S
Molecular Weight	239.68
CAS #	219929-01-8
SMILES	ClC1=CN=CC(=C1)OC(C1=CC=CS1)=O
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. High-throughput screening identifies inhibitors of the SARS coronavirus main proteinase. Chem Biol. 2004 Oct;11(10):1445-53. [2]. Lead compounds for the development of SARS-CoV-2 3CL protease inhibitors. Nat Commun. 2021 Apr 1;12(1):2016.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.1722 mL	20.8611 mL	41.7223 mL
	5 mM	0.8344 mL	4.1722 mL	8.3445 mL
	10 mM	0.4172 mL	2.0861 mL	4.1722 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.