Lignoceric acid (Tetracosanoic acid) is a 24-carbon saturated (24:0) fatty acid synthesized in the developing brain. Lignoceric acid is also a by-product of lignin production. Lignoceric acid may be utilized in the study of Zellweger's cerebral-hepatic-renal syndrome and adrenoleukodystrophy.

Physicochemical Properties

Molecular Formula	C24H48O2
Molecular Weight	368.64
Exact Mass	368.365
CAS #	557-59-5
Related CAS #	Lignoceric acid-d47;68060-00-4;Lignoceric acid-d4-1;1219794-59-8;Lignoceric acid-d4-2;1219794-61-2;Lignoceric acid-d9;2415226-90-1;Lignoceric acid-d3;851073-55-7
PubChem CID	11197
Appearance	White to off-white solid powder
Density	0.9±0.1 g/cm3
Boiling Point	405.9±8.0 °C at 760 mmHg
Melting Point	80-82 °C
Flash Point	182.2±13.3 °C
Vapour Pressure	0.0±1.0 mmHg at 25°C
Index of Refraction	1.460
LogP	11.4
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	22
Heavy Atom Count	26
Complexity	275
Defined Atom Stereocenter Count	0
InChi Key	QZZGJDVWLFXDLK-UHFFFAOYSA-N
InChi Code	InChI=1S/C24H48O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-21-22-23-24(25)26/h2-23H2,1H3,(H,25,26)
Chemical Name	tetracosanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Human Endogenous Metabolite
ln Vitro	Normal peroxisomal metabolism is shown by the exothermic heat flux in HepG2 peroxisomes produced by lignoceric acid [1].
References	[1]. Thermogenic flux induced by lignoceric acid in peroxisomes isolated from HepG2 cells and from X-adrenoleukodystrophy and control fibroblasts. J Cell Physiol. 2019 Aug;234(10):18344-18348. [2]. Lignoceric acid biosynthesis in the developing brain. Activities of mitochondrial acetyl-CoA-dependent synthesis and microsomal malonyl-CoA chain-elongating system in relation to myelination. Comparison between normal mouse and dysmyelinating mutants (quaking and jimpy). Eur J Biochem. 1977 Jan 3;72(1):41-7.
Additional Infomation	Tetracosanoic acid is a C24 straight-chain saturated fatty acid. It has a role as a volatile oil component, a plant metabolite, a human metabolite and a Daphnia tenebrosa metabolite. It is a very long-chain fatty acid and a straight-chain saturated fatty acid. It is a conjugate acid of a tetracosanoate. Tetracosanoic acid has been reported in Amaranthus hybridus, Tornabea scutellifera, and other organisms with data available. Lignoceric Acid is a saturated fatty acid with a 24-carbon backbone. Lignoceric acid occurs naturally in wood tar, various cerebrosides, and in small amount in most natural fats. lignoceric acid is a metabolite found in or produced by Saccharomyces cerevisiae.

Solubility Data

Solubility (In Vitro)

Ethanol :~8.33 mg/mL (~22.60 mM )

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 0.83 mg/mL (2.25 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear EtOH + stock solution to 900 μL of corn oil and mix well.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7127 mL	13.5634 mL	27.1267 mL
	5 mM	0.5425 mL	2.7127 mL	5.4253 mL
	10 mM	0.2713 mL	1.3563 mL	2.7127 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.