 (acetate) (Lactoferricin B acetate; Lfcin B acetate) Chemical Structure.png)
Physicochemical Properties
| Molecular Formula | C143H228N46O31S3 |
| Molecular Weight | 3183.83 |
| CAS # | 2828433-30-1 |
| Related CAS # | Lactoferrin (17-41);146897-68-9 |
| PubChem CID | 166450479 |
| Appearance | White to off-white solid powder |
| Hydrogen Bond Donor Count | 49 |
| Hydrogen Bond Acceptor Count | 43 |
| Rotatable Bond Count | 73 |
| Heavy Atom Count | 223 |
| Complexity | 6980 |
| Defined Atom Stereocenter Count | 26 |
| SMILES | C(=O)(O)C.C([C@@H]1NC(=O)C(NC([C@@]([H])(NC([C@H](CSSC[C@]([H])(NC([C@@]([H])(NC([C@@]([H])(NC([C@@H](NC([C@]2([H])CCCN2C(=O)[C@H](C)NC(=O)CNC(=O)[C@]([H])(CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@]([H])(CCCCN)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCCNC(N)=N)NC(=O)C(CC2=CNC3=CC=CC=C23)NC(=O)[C@H](CCC(=O)N)NC1=O)=O)CO)=O)[C@@H](C)CC)=O)[C@H](O)C)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)O)CC1C=CC=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CC1C=CC=CC=1)=O)CCCNC(N)=N)=O)CCCNC(N)=N)C1=CNC2=CC=CC=C12 |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | The minimum inhibitory concentration (MIC) of lactoferrin 17-41 (lacoferrin B) acetate against Escherichia coli ATCC 25922 is 30 μg/mL [1]. Lactoferrin 17–41 acetate exhibits cytotoxic action on HT-29 cells and dramatically increases HT-29 cell apoptosis [2]. To varied degrees, lactoferrin 17–41 acetate can control the transcription of genes such PMAIP-1, TP5313, and SFN that are implicated in the p53 signaling pathway[2]. Human monocyte LPS-induced cytokine responses can be inhibited by lactoferrin 17-41 acetate, which can bind LPS from Gram-negative bacteria [1][3]. |
| Cell Assay |
Cell Viability Assay[1] Cell Types: HT-29 cells Tested Concentrations: 50, 100, 200, 400, 800 or 1000 µg/mL Incubation Duration: 4, 12, 24 or 48 hrs (hours) Experimental Results: More effective at inducing apoptosis at 400 µg/mL. Higher toxicity is shown at 800 µg/mL. |
| References |
[1]. Anti-complement effects of lactoferrin-derived peptides. FEMS Immunol Med Microbiol. 2004 Jun 1;41(2):141-8. [2]. Bovine lactoferrin and lactoferricin exert antitumor activities on human colorectal cancer cells(HT-29) by activating various signaling pathways. Biochem Cell Biol. 2017 Feb;95(1):99-109. [3]. Reciprocal interactions between lactoferrin and bacterial endotoxins and their role in the regulation of the immune response. Toxins (Basel). 2010;2(1):54‐68. |
Solubility Data
| Solubility (In Vitro) | DMSO : 20 mg/mL (6.28 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2 mg/mL (0.63 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2 mg/mL (0.63 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2 mg/mL (0.63 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.3141 mL | 1.5704 mL | 3.1409 mL | |
| 5 mM | 0.0628 mL | 0.3141 mL | 0.6282 mL | |
| 10 mM | 0.0314 mL | 0.1570 mL | 0.3141 mL |