Lactitol monohydrate (CCRIS6315; Lactit; Lactosit Miruhen; NSC-231323; pizensy), the monohydrated form of lactitol and a disaccharide analogue of lactulose, is a naturally occuring sweetener and gastrointestinal agent as well as an osmotic laxative used for the treatment of chronic idiopathic constipation (CIC) in adults. It was approved in 2020 for medical uses as an oral solution to help produce bowel movements. In addition, it is also a reduced calorie sweetener derived from natural milk sugar, and has been used in a various sweetening applications.

Physicochemical Properties

Molecular Formula	C12H26O12
Molecular Weight	362.33
Exact Mass	362.142
CAS #	81025-04-9
Related CAS #	Lactitol;585-86-4
PubChem CID	3067270
Appearance	White to off-white solid powder
Density	1.69 g/cm3
Boiling Point	788.5ºC at 760 mmHg
Melting Point	95-98ºC
Flash Point	430.7ºC
Index of Refraction	1.634
Hydrogen Bond Donor Count	10
Hydrogen Bond Acceptor Count	12
Rotatable Bond Count	8
Heavy Atom Count	24
Complexity	343
Defined Atom Stereocenter Count	9
SMILES	O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1)[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)CO.O
InChi Key	LXMBXZRLTPSWCR-XBLONOLSSA-N
InChi Code	InChI=1S/C12H24O11.H2O/c13-1-4(16)7(18)11(5(17)2-14)23-12-10(21)9(20)8(19)6(3-15)22-12;/h4-21H,1-3H2;1H2/t4-,5+,6+,7+,8-,9-,10+,11+,12-;/m0./s1
Chemical Name	(2S,3R,4R,5R)-4-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhexane-1,2,3,5,6-pentol;hydrate
Synonyms	Lactitol monohydrate; D-Lactitol monohydrate; Lactitol (monohydrate); Lactitol hydrate; UH2K6W1Y64; DTXSID6047028
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Continuous combined intake of polydextrose and lactitol stimulates cecal fermentation and salivary IgA secretion in rats. J Oral Sci. 2017;59(4):603-610. [2]. Lactitol or lactulose in the treatment of chronic constipation: result of a systematic. J Indian Med Assoc. 2010 Nov;108(11):789-92./a >
Additional Infomation	See also: Lactitol (annotation moved to).

Solubility Data

Solubility (In Vitro)

H2O : ~120 mg/mL (~331.19 mM) DMSO : ~100 mg/mL (~275.99 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.90 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.90 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (6.90 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 25 mg/mL (69.00 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7599 mL	13.7996 mL	27.5991 mL
	5 mM	0.5520 mL	2.7599 mL	5.5198 mL
	10 mM	0.2760 mL	1.3800 mL	2.7599 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.