Physicochemical Properties
| Molecular Formula | C19H17FN4O3 |
| Molecular Weight | 368.36 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| Targets | HDAC6 |
| ln Vitro | LT-630 (2-8 nM, 3 hours and 24 hours) inhibits acetaminophen-induced cell damage and reduces increased oxidative stress and apoptosis in AML-12 cells [1]. |
| ln Vivo | LT-630 (10-40 mg/kg, intravenous, before APAP administration) inhibits acetaminophen (APAP)-induced oxidative stress and hepatocyte apoptosis in mice by eliminating ROS [1]. |
| Cell Assay |
Western Blot Analysis[1] Cell Types: AML-12 cells Tested Tested Concentrations: 2-8 nM Incubation Duration: 3 h and 24 h Experimental Results: Reduced the increased ratio of bax/bcl-2 and the levels of cleaved caspase3 and cyt-c. |
| Animal Protocol |
Animal/Disease Models: male C57BL/6J mice (16–20 g, 6-week-old)[1] Doses: 10-40 mg/kg befor APAP (350 mg/kg, i.p.) Route of Administration: tail vein injection Experimental Results: Enhanced the release of GSH, SOD and NADPH. Reduced apoptosis, the bax/bcl-2 ratio, and the levels of cleaved caspase3 and cyt-c in APAP. |
| References |
[1]. A novel HDAC6 inhibitor attenuate APAP-induced liver injury by regulating MDH1-mediated oxidative stress. Int Immunopharmacol. 2024 Mar 13;131:111861. |
Solubility Data
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7147 mL | 13.5737 mL | 27.1474 mL | |
| 5 mM | 0.5429 mL | 2.7147 mL | 5.4295 mL | |
| 10 mM | 0.2715 mL | 1.3574 mL | 2.7147 mL |