LT-630 is an HDAC6 inhibitor that alleviates liver damage by reducing oxidative stress.

Physicochemical Properties

Molecular Formula	C19H17FN4O3
Molecular Weight	368.36
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	HDAC6
ln Vitro	LT-630 (2-8 nM, 3 hours and 24 hours) inhibits acetaminophen-induced cell damage and reduces increased oxidative stress and apoptosis in AML-12 cells [1].
ln Vivo	LT-630 (10-40 mg/kg, intravenous, before APAP administration) inhibits acetaminophen (APAP)-induced oxidative stress and hepatocyte apoptosis in mice by eliminating ROS [1].
Cell Assay	Western Blot Analysis[1] Cell Types: AML-12 cells Tested Tested Concentrations: 2-8 nM Incubation Duration: 3 h and 24 h Experimental Results: Reduced the increased ratio of bax/bcl-2 and the levels of cleaved caspase3 and cyt-c.
Animal Protocol	Animal/Disease Models: male C57BL/6J mice (16–20 g, 6-week-old)[1] Doses: 10-40 mg/kg befor APAP (350 mg/kg, i.p.) Route of Administration: tail vein injection Experimental Results: Enhanced the release of GSH, SOD and NADPH. Reduced apoptosis, the bax/bcl-2 ratio, and the levels of cleaved caspase3 and cyt-c in APAP.
References	[1]. A novel HDAC6 inhibitor attenuate APAP-induced liver injury by regulating MDH1-mediated oxidative stress. Int Immunopharmacol. 2024 Mar 13;131:111861.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7147 mL	13.5737 mL	27.1474 mL
	5 mM	0.5429 mL	2.7147 mL	5.4295 mL
	10 mM	0.2715 mL	1.3574 mL	2.7147 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.