LEESGGGLVQPGGSMK acetate, a proteolytic peptide that is a component of Infliximab, may be utilized in the quantitative analysis of Infliximab. Infliximab is a chimeric monoclonal IgG1 antibody that specifically binds to TNF-α.

Physicochemical Properties

Molecular Formula	C66H112N18O26S
Molecular Weight	1605.76
Related CAS #	LEESGGGLVQPGGSMK;2096980-79-7;LEESGGGLVQPGGSMK TFA
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

Anti-TNF antibodies obstruct the effects of TNF alpha, a novel therapy for TNF-related disorders, including rheumatoid arthritis, psoriatic arthritis, ulcerative colitis, ankylosing spondylitis, and inflammatory bowel disease. Anti-TNF antibodies stimulate mucosal healing and long-term remissions in vivo by blocking TNF activity. The primary anti-TNF antibodies that are now approved include Golimumab, Adalimumab, Certolizumab, Etanercept, and Infliximab[1].

References

[1]. A method for quantifying therapeutic antibodies. EP3371592A1.

Solubility Data

Solubility (In Vitro)	H2O :~100 mg/mL (~62.28 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (62.28 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.6228 mL	3.1138 mL	6.2276 mL
	5 mM	0.1246 mL	0.6228 mL	1.2455 mL
	10 mM	0.0623 mL	0.3114 mL	0.6228 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.