L-Cystine diHCl can be used as a cell culture component and is a sulfur-containing precursor for the synthesis of glutathione (GSH). Homeostasis of L-Cystine diHCl is also important for glutathione function.

Physicochemical Properties

Molecular Formula	C6H14CL2N2O4S2
Molecular Weight	311.20648
Exact Mass	311.977
CAS #	30925-07-6
PubChem CID	21121987
Appearance	White to off-white solid powder
Boiling Point	505.5ºC at 760 mmHg
Flash Point	259.5ºC
LogP	2.196
Hydrogen Bond Donor Count	6
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	7
Heavy Atom Count	16
Complexity	192
Defined Atom Stereocenter Count	2
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Thioredoxin-related protein of 14 kDa is an efficient L-cystine reductase and S-denitrosylase. Proc Natl Acad Sci U S A. 2014 May 13;111(19):6964-9. [2]. Xiaojun Lian. Serum-free human pluripotent stem cell culture medium. Patent WO2021094826 A1.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~159.63 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (3.99 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.2133 mL	16.0663 mL	32.1326 mL
	5 mM	0.6427 mL	3.2133 mL	6.4265 mL
	10 mM	0.3213 mL	1.6066 mL	3.2133 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.