L-Cysteine HCl hydrate is a conditionally essential amino acid (AA) and a precursor to bioactive molecules such as hydrogen sulfide (H2S), glutathione and taurine. L-Cysteine HCl hydrate inhibits ghrelin and reduces appetite in rodents and humans.

Physicochemical Properties

Molecular Formula	C3H10CLNO3S
Molecular Weight	175.63
Exact Mass	175.006
CAS #	7048-04-6
Related CAS #	L-Cysteine;52-90-4;L-Cysteine hydrochloride;52-89-1
PubChem CID	23462
Appearance	White to off-white solid powder
Boiling Point	293.9ºC at 760 mmHg
Melting Point	175°C
Flash Point	131.5ºC
Index of Refraction	6 ° (C=8, 1mol/L HCl)
LogP	0.766
Hydrogen Bond Donor Count	5
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	2
Heavy Atom Count	9
Complexity	75.3
Defined Atom Stereocenter Count	1
SMILES	C([C@@H](C(=O)O)N)S.O.Cl
InChi Key	QIJRTFXNRTXDIP-JIZZDEOASA-N
InChi Code	InChI=1S/C3H7NO2S.ClH.H2O/c4-2(1-7)3(5)6;;/h2,7H,1,4H2,(H,5,6);1H;1H2/t2-;;/m0../s1
Chemical Name	(2R)-2-amino-3-sulfanylpropanoic acid;hydrate;hydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Microbial Metabolite Human Endogenous Metabolite
References	[1]. L-cysteine suppresses ghrelin and reduces appetite in rodents and humans. Int J Obes (Lond). 2015 Mar;39(3):447-55.
Additional Infomation	L-cysteine hydrochloride hydrate is a hydrate that is the monohydrate form of L-cysteine hydrochloride. It has a role as an EC 4.3.1.3 (histidine ammonia-lyase) inhibitor, a flour treatment agent and a human metabolite. It contains a L-cysteine hydrochloride. See also: Cysteine Hydrochloride (annotation moved to).

Solubility Data

Solubility (In Vitro)

DMSO :~100 mg/mL (~569.38 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (14.23 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.23 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.6938 mL	28.4689 mL	56.9379 mL
	5 mM	1.1388 mL	5.6938 mL	11.3876 mL
	10 mM	0.5694 mL	2.8469 mL	5.6938 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.