L-2-Hydroxyglutaric acid disodium is an epigenetic modifier that may be utilized in renal cancer-related research. L-2-Hydroxyglutaric acid disodium inhibits histone demethylases, thereby promoting histone methylation. L-2-Hydroxyglutaric acid inhibits mitochondrial creatine kinase (Mi-CK) activity, with Km and Ki of 2.52 mM and 11.13 mM respectively.

Physicochemical Properties

Molecular Formula	C5H6O5-2.2[NA+]
Molecular Weight	192.07774
Exact Mass	192.001
CAS #	63512-50-5
Related CAS #	L-2-Hydroxyglutaric acid;13095-48-2
PubChem CID	56845267
Appearance	White to off-white solid powder
Melting Point	>300ºC
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	2
Heavy Atom Count	12
Complexity	130
Defined Atom Stereocenter Count	1
SMILES	C(CC(=O)[O-])[C@@H](C(=O)[O-])O.[Na+].[Na+]
InChi Key	DZHFTEDSQFPDPP-QTNFYWBSSA-L
InChi Code	InChI=1S/C5H8O5.2Na/c6-3(5(9)10)1-2-4(7)8;;/h3,6H,1-2H2,(H,7,8)(H,9,10);;/q;2*+1/p-2/t3-;;/m0../s1
Chemical Name	disodium;(2S)-2-hydroxypentanedioate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Eleven-eleven translocation (TET) enzymes and other 2-oxoglutarate (2-OG)-dependent dioxygenases (2OGD) are efficiently inhibited by L-2-hydroxyglutarate [1].
References	[1]. L-2-Hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer. Cancer Discov. 2014 Nov;4(11):1290-8. [2]. L-2-hydroxyglutaric acid inhibits mitochondrial creatine kinase activity from cerebellum of developing rats. Int J Dev Neurosci. 2003 Jun;21(4):217-24.

Solubility Data

Solubility (In Vitro)	H2O : ~83.33 mg/mL (~433.83 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 25 mg/mL (130.15 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with heating and sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.2062 mL	26.0308 mL	52.0616 mL
	5 mM	1.0412 mL	5.2062 mL	10.4123 mL
	10 mM	0.5206 mL	2.6031 mL	5.2062 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.