KIN101 is an effective RNA viral inhibitor that has IC50 values of 2 μM and >5 μM for Dengue virus (DNV) and influenza virus, respectively. Nuclear translocation of IRF-3 is induced by KIN101, an isoflavone agonist of IRF-3 dependent signaling. KIN101 exhibits broad-spectrum anti-RNA virus activity.

Physicochemical Properties

Molecular Formula	C16H11BRO5S
Molecular Weight	395.2245
Exact Mass	393.95
Elemental Analysis	C, 48.62; H, 2.81; Br, 20.22; O, 20.24; S, 8.11
CAS #	610753-87-2
PubChem CID	1998840
Appearance	White to off-white solid powder
LogP	3.4
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	23
Complexity	586
Defined Atom Stereocenter Count	0
SMILES	BrC1C([H])=C([H])C(=C([H])C=1[H])C1=C([H])OC2C([H])=C(C([H])=C([H])C=2C1=O)OS(C([H])([H])[H])(=O)=O
InChi Key	SJGDYHHAYHRLNC-UHFFFAOYSA-N
InChi Code	InChI=1S/C16H11BrO5S/c1-23(19,20)22-12-6-7-13-15(8-12)21-9-14(16(13)18)10-2-4-11(17)5-3-10/h2-9H,1H3
Chemical Name	3-(4-bromophenyl)-4-oxo-4H-chromen-7-yl methanesulfonate
Synonyms	KIN101; KIN 101; KIN-101
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	influenza virus(IC50= 2 μM);DNV(IC50>5 μM)
ln Vitro	Significant reduction in the abundance of NP protein is observed when exposed to KIN 101 (10 μM) for 24 hours.The HCV RNA levels in KIN 101 (10 μM; 18 hours) decrease by more than one log[1]. KIN 101 (0.01, 0.1, 1, 10, 100 μM) has an IC50 of 0.2 μM and significantly and dose-dependently affects the formation of foci.In MRC5 cells, KIN 101 (5, 10, 20, 50 μM; 4 hours) reduces influenza virus infection in a dose-dependent manner.ISG levels, as well as those of other proteins like RIG-I and MDA5 that are activated downstream of IRF, are significantly elevated by KIN 101[1]. The antiviral activity of KIN101 (0.1-100 μM; 18 hours) is demonstrated against the hepatitis C virus (HCV)[2].
References	[1]. Isoflavone agonists of IRF-3 dependent signaling have antiviral activity against RNA viruses. J Virol. 2012 Jul;86(13):7334-44. [2]. Anti-viral compounds, pharmaceutical compositions and methods of use thereof. US20160122312A1.

Solubility Data

Solubility (In Vitro)

DMSO : ~41.67 mg/mL (~105.43 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (5.26 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.5302 mL	12.6512 mL	25.3024 mL
	5 mM	0.5060 mL	2.5302 mL	5.0605 mL
	10 mM	0.2530 mL	1.2651 mL	2.5302 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.