KH7 (KH-7) is a novel and potent Inhibitor of Sac (soluble adenylyl cyclase) with IC50s of 3-10 μM against both recombinant purified human sACt protein and heterologously expressed sACt in cellular assays. It is also a cAMP inhibitor.

Physicochemical Properties

Molecular Formula	C17H15BRN4O2S
Molecular Weight	419.297
Exact Mass	418.009
Elemental Analysis	C, 48.70; H, 3.61; Br, 19.06; N, 13.36; O, 7.63; S, 7.65
CAS #	330676-02-3
PubChem CID	135496218
Appearance	White to light yellow solid powder
Density	1.6±0.1 g/cm3
Index of Refraction	1.716
LogP	6.01
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	5
Heavy Atom Count	25
Complexity	495
Defined Atom Stereocenter Count	0
SMILES	CC(C(N/N=C/C1=C(O)C=CC(Br)=C1)=O)SC1NC2=C(C=CC=C2)N=1
InChi Key	WILMXUAKQKGGCC-DJKKODMXSA-N
InChi Code	InChI=1S/C17H15BrN4O2S/c1-10(25-17-20-13-4-2-3-5-14(13)21-17)16(24)22-19-9-11-8-12(18)6-7-15(11)23/h2-10,23H,1H3,(H,20,21)(H,22,24)/b19-9+
Chemical Name	2-(1H-benzimidazol-2-ylsulfanyl)-N-[(E)-(5-bromo-2-hydroxyphenyl)methylideneamino]propanamide
Synonyms	KH-7; KH7; KH 7
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	recombinant sACt ( IC50 = 3-10 μM )
ln Vitro	KH7 (10 μM) inhibits this increase in cAMP caused by capture energy. In beer, KH7 significantly reduces basal cAMP accumulation, regardless of incubation medium, at higher concentrations (50 μM), 5 to 10 times higher than its IC50 and still selective against tmAC and sAC [1]. KH7 inhibits the synthesis of CaSF [2]. Myocytes exhibit a 20% negative inotropic effect (NIE) when KH7 is present, suggesting a role for sAC in the development of basal myocardial contractility [2].
References	[1]. The "soluble" adenylyl cyclase in sperm mediates multiple signaling events required for fertilization. Dev Cell. 2005 Aug;9(2):249-59. [2]. Maresin Conjugates in Tissue Regeneration 1 improves alveolar fluid clearance by up-regulating alveolar ENaC, Na, K-ATPase in lipopolysaccharide-induced acute lung injury. 4.658J Cell Mol Med. 2020 Mar 11.

Solubility Data

Solubility (In Vitro)

DMSO: ~250 mg/mL (~596.2 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (5.96 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.96 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (4.96 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.3849 mL	11.9246 mL	23.8493 mL
	5 mM	0.4770 mL	2.3849 mL	4.7699 mL
	10 mM	0.2385 mL	1.1925 mL	2.3849 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.