JohnPhos is a biochemical compound that could be utilized as a biomaterial or organic/chemical reagent for life science related research.

Physicochemical Properties

Molecular Formula	C20H27P
Molecular Weight	298.40
Exact Mass	298.185
CAS #	224311-51-7
PubChem CID	2734215
Appearance	White to off-white solid powder
Density	1 g/cm3
Boiling Point	405.5±24.0 °C at 760 mmHg
Melting Point	86-88 °C(lit.)
Flash Point	210.1±29.2 °C
Vapour Pressure	0.0±0.9 mmHg at 25°C
LogP	6.78
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	0
Rotatable Bond Count	4
Heavy Atom Count	21
Complexity	300
Defined Atom Stereocenter Count	0
InChi Key	CNXMDTWQWLGCPE-UHFFFAOYSA-N
InChi Code	InChI=1S/C20H27P/c1-19(2,3)21(20(4,5)6)18-15-11-10-14-17(18)16-12-8-7-9-13-16/h7-15H,1-6H3
Chemical Name	ditert-butyl-(2-phenylphenyl)phosphane
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Solubility Data

Solubility (In Vitro)

DMSO: 10 mg/mL (33.51 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1 mg/mL (3.35 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.3512 mL	16.7560 mL	33.5121 mL
	5 mM	0.6702 mL	3.3512 mL	6.7024 mL
	10 mM	0.3351 mL	1.6756 mL	3.3512 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.